Invitrogen

GST Tag Monoclonal Antibody (8-326), HRP

FIGURE: 1 / 6

GST Tag Antibody (MA4-004-HRP) in WB

Western blot analysis of GST was performed by loading various amounts of purified GST-SRC SH2 domain per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GST monoclonal antibody (Product # MA4-004-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080) and th... View More

GST Tag Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

MA4-004-HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

GST Pull Down (GPD)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8-326

Immunogen

Recombinant purified full-length GST

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.07% Kathon

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537634

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting pleckstrin-2/Akt signaling reduces proliferation in myeloproliferative neoplasm models.

The Journal of clinical investigation

Han X,Mei Y,Mishra RK,Bi H,Jain AD,Schiltz GE,Zhao B,Sukhanova M,Wang P,Grigorescu AA,Weber PC,Piwinski JJ,Prado MA,Paulo JA,Stephens L,Anderson KE,Abrams CS,Yang J,Ji P

MA4-004-HRP was used in Western Blotting to reveal a Plek2-Akt complex that drives cell proliferation and can be targeted by a new class of anti-proliferative compounds for MPN therapy.

Wed Mar 15 00:00:00 EDT 2023

Initiation of Parkinson's disease from gut to brain by δ-secretase.

Cell research

Ahn EH,Kang SS,Liu X,Chen G,Zhang Z,Chandrasekharan B,Alam AM,Neish AS,Cao X,Ye K

MA4-004-HRP was used in GST Pull Down to conclude that δ-secretase plays a crucial role in initiating PD pathology progression from the ENS to the CNS.

Wed Jan 01 00:00:00 EST 2020

MDMX inhibits casein kinase 1α activity and stimulates Wnt signaling.

The EMBO journal

Huang Q,Chen L,Schonbrunn E,Chen J

MA4-004-HRP was used in Western Blot to investigate if MDMX is a potent competitive inhibitor of casein kinase 1 alpha kinase activity (Ki = 8 nM).

Wed Jul 15 00:00:00 EDT 2020

Nuclear Import of JAK1 Is Mediated by a Classical NLS and Is Required for Survival of Diffuse Large B-cell Lymphoma.

Molecular cancer research : MCR

Zhu F,Hwang B,Miyamoto S,Rui L

Published figure using GST Tag monoclonal antibody (Product # MA4-004-HRP) in Western Blot

Wed Mar 01 00:00:00 EST 2017

Primary angle closure glaucoma (PACG) susceptibility gene PLEKHA7 encodes a novel Rac1/Cdc42 GAP that modulates cell migration and blood-aqueous barrier function.

Human molecular genetics

Lee MC,Shei W,Chan AS,Chua BT,Goh SR,Chong YF,Hilmy MH,Nongpiur ME,Baskaran M,Khor CC,Aung T,Hunziker W,Vithana EN

MA4-004-HRP was used in Western Blotting to conclude that downregulation of PLEKHA7 in PACG may affect BAB integrity and aqueous humor outflow via its Rac1/Cdc42 GAP activity, thereby contributing to disease etiology.

Sun Oct 15 00:00:00 EDT 2017

Rafoxanide and Closantel Inhibit SPAK and OSR1 Kinases by Binding to a Highly Conserved Allosteric Site on Their C-terminal Domains.

ChemMedChem

AlAmri MA,Kadri H,Alderwick LJ,Simpkins NS,Mehellou Y

MA4004HRP was used in western blot to identify and study the allosteric pockets of SPAK and OSR1

Tue May 09 00:00:00 EDT 2017

NKD Transcription Factors Are Central Regulators of Maize Endosperm Development.

The Plant cell

Gontarek BC,Neelakandan AK,Wu H,Becraft PW

MA4-004-HRP was used in western blot to investigate the roles of NKD1 and NKD2 in maize endosperm development

Thu Dec 01 00:00:00 EST 2016

E-cadherin-mediated force transduction signals regulate global cell mechanics.

Journal of cell science

Muhamed I,Wu J,Sehgal P,Kong X,Tajik A,Wang N,Leckband DE

Published figure using GST Tag monoclonal antibody (Product # MA4-004-HRP) in Western Blot

Sun May 01 00:00:00 EDT 2016

A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA-MB-231.

Oncology letters

Liu WQ,Yang J,Hong M,Gao CE,Dong J

Published figure using GST Tag monoclonal antibody (Product # MA4-004-HRP) in Western Blot

Wed Jun 01 00:00:00 EDT 2016

A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA-MB-231.

Oncology letters

Liu WQ,Yang J,Hong M,Gao CE,Dong J

Published figure using GST Tag monoclonal antibody (Product # MA4-004-HRP) in Western Blot

Wed Jun 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GST Tag Monoclonal Antibody (8-326), HRP

GST Tag Antibody (MA4-004-HRP) in WB

Product Details

MA4-004-HRP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

GST Pull Down (GPD)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!