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FIGURE: 1 / 1
GTF2F2 Antibody (MA5-53056) in WB
Western Blot analysis of GTF2F2. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF2F2 recombinant monoclonal antibody (Product # MA5-53056) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
Product Details
MA5-53056
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB6490
Immunogen
A synthesized peptide derived from GTF2F2 (1-29AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
GTF2F2 is required for both basal and HIV-1 Tat-activated transcription. GTF2F2 synergizes with HIV-1 Tat and the cellular co-activator Tat-SF1 during Tat-mediated transactivation of the HIV-1 LTR promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; General transcription factor IIF subunit 2; general transcription factor IIF, polypeptide 2 (30kD subunit); general transcription factor IIF, polypeptide 2, 30kDa; TFIIF beta; TFIIF-beta; transcription factor gamma; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30
Gene Aliases: 1110031C13Rik; AI326315; BTF4; GTF2F2; RAP30; TF2F2; TFIIF
UniProt ID: (Human) P13984, (Mouse) Q8R0A0
Entrez Gene ID: (Human) 2963, (Mouse) 68705, (Rat) 81674
mRNA splicing, via spliceosome
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
fibroblast growth factor receptor signaling pathway
gene expression
positive regulation of transcription elongation from RNA polymerase II promoter
snRNA transcription from RNA polymerase II promoter
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of viral transcription
positive regulation of transcription initiation from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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