Proteintech

Galectin-3 Polyclonal Antibody

Galectin-3 Antibody in Immunocytochemistry (ICC/IF)

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Galectin-3 Antibody (14979-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 14979-1-AP (Galectin-3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Galectin-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Galectin-3 Antibody in Western Blot (WB)

Galectin-3 Antibody in Flow Cytometry (Flow)

Galectin-3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14979-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 9 publications 9 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Galectin-3 Fusion Protein Ag6891 (1-250 aa encoded by BC001120)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADNFSLHD ALSGSGNPNP QGWPGAWGNQ PAGAGGYPGA SYPGAYPGQA PPGAYPGQAP PGAYPGAPGA YPGAPAPGVY PGPPSGPGAY PSSGQPSATG AYPATGPYGA PAGPLIVPYN LPLPGGVVPR MLITILGTVK PNANRIALDF QRGNDVAFHF NPRFNENNRR VIVCNTKLDN NWGREERQSV FPFESGKPFK IQVLVEPDHF KVAVNDAHLL QYNHRVKKLN EISKLGISGD IDLTSASYTM I (1-250 aa encoded by BC001120)

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption.

The Journal of cell biology

Zhu L,Tang Y,Li XY,Kerk SA,Lyssiotis CA,Sun X,Wang Z,Cho JS,Ma J,Weiss SJ

Mon Apr 03 00:00:00 EDT 2023

ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface.

Oncogene

Zou X,Lu J,Deng Y,Liu Q,Yan X,Cui Y,Xiao X,Fang M,Yang F,Sawaki H,Sato T,Tan B,Lu X,Feng B,Kuno A,Narimatsu H,Gao C,Zhang Y

Wed Feb 01 00:00:00 EST 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

Inhibition of galectin-3 ameliorates high-glucose-induced oxidative stress and inflammation in ARPE-19 cells.

Cutaneous and ocular toxicology

Li M,Tian M,Jiang X,Liu Y,Wang Y,Li Y

Wed Jun 01 00:00:00 EDT 2022

A phosphoinositide signalling pathway mediates rapid lysosomal repair.

Nature

Tan JX,Finkel T

Thu Sep 01 00:00:00 EDT 2022

Protection of CAPE-pNO₂ Against Chronic Myocardial Ischemia by the TGF-Β1/Galectin-3 Pathway In Vivo and In Vitro.

Inflammation

Wan Q,Zhang L,Zhou Q,Han Y,Li Z,Li B

Wed Jun 01 00:00:00 EDT 2022

Protection of CAPE-pNO₂ Against Chronic Myocardial Ischemia by the TGF-Β1/Galectin-3 Pathway In Vivo and In Vitro.

Inflammation

Wan Q,Zhang L,Zhou Q,Han Y,Li Z,Li B

Wed Jun 01 00:00:00 EDT 2022

Protection of CAPE-pNO₂ Against Chronic Myocardial Ischemia by the TGF-Β1/Galectin-3 Pathway In Vivo and In Vitro.

Inflammation

Wan Q,Zhang L,Zhou Q,Han Y,Li Z,Li B

Wed Jun 01 00:00:00 EDT 2022

Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production.

Cellular signalling

Pan D,Wu W,Zuo G,Xie X,Li H,Ren X,Kong C,Zhou W,Zhang Z,Waterfall M,Chen S

Sat Oct 01 00:00:00 EDT 2022

Galectin-3 inhibition boosts the therapeutic efficacy of Semliki Forest virus in pediatric osteosarcoma.

Molecular therapy oncolytics

Herrador-Cañete G,Zalacain M,Labiano S,Laspidea V,Puigdelloses M,Marrodan L,Garcia-Moure M,Gonzalez-Huarriz M,Marco-Sanz J,Ausejo-Mauleon I,de la Nava D,Hernández-Osuna R,Martínez-García J,Silva-Pilipich N,Gurucega E,Patiño-García A,Hernández-Alcoceba R,Smerdou C,Alonso MM

Thu Sep 15 00:00:00 EDT 2022

Prognostic Value and Biological Function of Galectins in Malignant Glioma.

Frontiers in oncology

Zhu H,Liu D,Cheng L,Liu J,Wang G,Li H,Zhang Y,Mi H,Zhang S,Shu K,Yu X

Sat Jul 16 00:00:00 EDT 2022

Prognostic Value and Biological Function of Galectins in Malignant Glioma.

Frontiers in oncology

Zhu H,Liu D,Cheng L,Liu J,Wang G,Li H,Zhang Y,Mi H,Zhang S,Shu K,Yu X

Sat Jul 16 00:00:00 EDT 2022

Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis.

International journal of biological sciences

Li Z,Wang T,Xin C,Song Y,Kong J,Xu J,Liu Q,Teng Y,Hou N,Cheng X,Yang G,Liu W,Zhou B,Zhang Y,Yang X,Wang J

Fri Dec 23 00:00:00 EST 2022

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model.

Scientific reports

Herrera-Marcos LV,Martínez-Beamonte R,Macías-Herranz M,Arnal C,Barranquero C,Puente-Lanzarote JJ,Gascón S,Herrero-Continente T,Gonzalo-Romeo G,Alastrué-Vera V,Gutiérrez-Blázquez D,Lou-Bonafonte JM,Surra JC,Rodríguez-Yoldi MJ,García-Gil A,Güemes A,Osada J

Wed Jan 19 00:00:00 EST 2022

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model.

Scientific reports

Wed Jan 19 00:00:00 EST 2022

Substitution of the SERCA2 Cys⁶⁷⁴ reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation.

British journal of pharmacology

Su H,Mei Y,Luo S,Wu H,He Y,Shiraishi Y,Hu P,Cohen RA,Tong X

Sat Oct 01 00:00:00 EDT 2022

Galectin-3 mediates cardiac remodeling caused by impaired glucose and lipid metabolism through inhibiting two pathways of activating Akt.

American journal of physiology. Heart and circulatory physiology

Sun Z,Zhang L,Li L,Shao C,Liu J,Zhou M,Wang Z

Fri Jan 01 00:00:00 EST 2021

Galectin-3 mediates cardiac remodeling caused by impaired glucose and lipid metabolism through inhibiting two pathways of activating Akt.

American journal of physiology. Heart and circulatory physiology

Sun Z,Zhang L,Li L,Shao C,Liu J,Zhou M,Wang Z

Fri Jan 01 00:00:00 EST 2021

Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation.

Cardiology

Xiao M,Zhang M,Bie M,Wang X,Guo J,Xiao H

Mon Apr 12 00:00:00 EDT 2021

Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation.

Cardiology

Xiao M,Zhang M,Bie M,Wang X,Guo J,Xiao H

Mon Apr 12 00:00:00 EDT 2021

Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation.

Cardiology

Xiao M,Zhang M,Bie M,Wang X,Guo J,Xiao H

Mon Apr 12 00:00:00 EDT 2021

Assessment of mitochondrial function in metabolic dysfunction-associated fatty liver disease using obese mouse models.

Zoological research

Zhao QY,Ge LH,Zhang K,Chen HF,Zhan XX,Yang Y,Dang QL,Zheng Y,Zhou HB,Lyu JX,Fang HZ

Fri Sep 18 00:00:00 EDT 2020

Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure.

Virus research

Nakamura H,Shimizu T,Takatani A,Suematsu T,Nakamura T,Kawakami A

Thu Aug 01 00:00:00 EDT 2019

Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1.

Frontiers in cell and developmental biology

Li Z,Lv F,Dai C,Wang Q,Jiang C,Fang M,Xu Y

Thu Oct 01 00:00:00 EDT 2020

Galectin-3 Mediates Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension.

Aging and disease

Li T,Zha L,Luo H,Li S,Zhao L,He J,Li X,Qi Q,Liu Y,Yu Z

Thu Aug 01 00:00:00 EDT 2019

Galectin-3 Mediates Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension.

Aging and disease

Li T,Zha L,Luo H,Li S,Zhao L,He J,Li X,Qi Q,Liu Y,Yu Z

Thu Aug 01 00:00:00 EDT 2019

Synephrine Hydrochloride Suppresses Esophageal Cancer Tumor Growth and Metastatic Potential through Inhibition of Galectin-3-AKT/ERK Signaling.

Journal of agricultural and food chemistry

Xu WW,Zheng CC,Huang YN,Chen WY,Yang QS,Ren JY,Wang YM,He QY,Liao HX,Li B

Wed Sep 05 00:00:00 EDT 2018

Synephrine Hydrochloride Suppresses Esophageal Cancer Tumor Growth and Metastatic Potential through Inhibition of Galectin-3-AKT/ERK Signaling.

Journal of agricultural and food chemistry

Xu WW,Zheng CC,Huang YN,Chen WY,Yang QS,Ren JY,Wang YM,He QY,Liao HX,Li B

Wed Sep 05 00:00:00 EDT 2018

Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension.