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Invitrogen
Galectin 3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
Galectin 3 Antibody (PA5-34819) in ELISA
Sandwich ELISA detection of recombinant full-length Galectin 3 protein using a Galectin 3 Monoclonal Antibody as capture antibody at concentration of 5 µg/mL and Galectin 3 Polyclonal Antibody (Product # PA5-34819) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10,000 and used to detect the primary antibody.
Product Details
PA5-34819
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 184 of Galectin3 (Uniprot ID#P17931)
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552171
Product Specific Information
Recommended positive controls: HeLa, A549, H1299, HCT116, MCF-7, Recombinant LGALS3 protein, rat colon.
Predicted reactivity: Rhesus Monkey (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; beta-galactosides-binding lectin; Carbohydrate-binding protein 35; CBP 35; CBP-30; epsilon BP; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE binding protein; IgE-binding protein; L-29; L-31; Laminin-binding protein; lectin galactoside-binding soluble 3 protein; Lectin L-29; lectin, galactose binding, soluble 3; lectin, galactoside-binding, soluble, 3; lectin, galactoside-binding, soluble, 3 (galectin 3); MAC-2 antigen
Gene Aliases: AGE-R3; CBP 35; CBP30; CBP35; Gal-3; GAL3; GALBP; Galectin-3; GALIG; L-34; L31; LGALS2; LGALS3; MAC2
UniProt ID: (Human) Q96J47, (Rat) P08699
Entrez Gene ID: (Human) 3958, (Dog) 404021, (Rat) 83781
monocyte chemotaxis
mRNA processing
RNA splicing
neutrophil chemotaxis
epithelial cell differentiation
regulation of T cell proliferation
innate immune response
negative regulation of endocytosis
eosinophil chemotaxis
macrophage chemotaxis
negative regulation of T cell receptor signaling pathway
positive chemotaxis
regulation of T cell apoptotic process
mononuclear cell migration
positive regulation of mononuclear cell migration
positive regulation of calcium ion import
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
positive regulation of protein complex assembly
positive regulation of protein localization to plasma membrane
skeletal system development
signal transduction
positive regulation of cell proliferation
positive regulation of serotonin secretion
extracellular matrix organization
negative regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell proliferation in bone marrow
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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