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Invitrogen
Galectin 3 Recombinant Rabbit Monoclonal Antibody (024)
FIGURE: 1 / 4
Galectin 3 Antibody (MA5-29390) in ICC/IF
Immunofluorescence staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Galectin 3 Recombinant Rabbit Monoclonal Antibody (24) (Product # MA5-29390, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was lo... View More
Product Details
MA5-29390
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
024
Immunogen
Recombinant Human Galectin-3/LGALS3 protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785260
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Galectin-3/LGALS3.
Target Information
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; MAC-2 antigen
Gene Aliases: CBP35; GAL3; GALBP; GALIG; L31; LGALS2; LGALS3; MAC2
UniProt ID: (Human) Q96J47
Entrez Gene ID: (Human) 3958
monocyte chemotaxis
mRNA processing
RNA splicing
neutrophil chemotaxis
epithelial cell differentiation
regulation of T cell proliferation
innate immune response
negative regulation of endocytosis
eosinophil chemotaxis
macrophage chemotaxis
negative regulation of T cell receptor signaling pathway
positive chemotaxis
regulation of T cell apoptotic process
mononuclear cell migration
positive regulation of mononuclear cell migration
positive regulation of calcium ion import
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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