Invitrogen

Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eFluor™ 660, eBioscience™

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Antibody Testing Data (1)
Published Figures (36)

Galectin 3 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 37

Galectin 3 Antibody (50-5301-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.06 µg of Anti-Human/Mouse Galectin-3 eFluor® 660 (right). Cells in the large scatter population were used for analysis.

Galectin 3 Antibody in Immunocytochemistry (ICC/IF)

Galectin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Galectin 3 Antibody in Immunohistochemistry (IHC)

Galectin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Galectin 3 Antibody in Western Blot (WB)

Galectin 3 Antibody in Western Blot, Flow Cytometry (WB, Flow)

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Product Details

50-5301-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 18 publications 18 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.125 µg/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioM3/38 (M3/38)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11220276

Product Specific Information

Description: The eBioM3/38 (M3/38) antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Proteolytic processing of galectin-3 by meprin metalloproteases is crucial for host-microbiome homeostasis.

Science advances

Bülck C,Nyström EEL,Koudelka T,Mannbar-Frahm M,Andresen G,Radhouani M,Tran F,Scharfenberg F,Schrell F,Armbrust F,Dahlke E,Zhao B,Vervaeke A,Theilig F,Rosenstiel P,Starkl P,Rosshart SP,Fickenscher H,Tholey A,Hansson GC,Becker-Pauly C

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Western Blot

Fri Mar 31 00:00:00 EDT 2023

Human granzyme B regulatory B cells prevent effector CD4+CD25- T cell proliferation through a mechanism dependent from lymphotoxin alpha.

Frontiers in immunology

Sailliet N,Mai HL,Dupuy A,Tilly G,Fourgeux C,Braud M,Giral M,Robert JM,Degauque N,Danger R,Poschmann J,Brouard S

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Western Blot

Fri Aug 18 00:00:00 EDT 2023

Characterization and Quantitation of the Tumor Microenvironment of Uveal Melanoma.

Biology

Goesmann L,Refaian N,Bosch JJ,Heindl LM

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Fri May 19 00:00:00 EDT 2023

Modulating sphingosine 1-phosphate receptor signaling skews intrahepatic leukocytes and attenuates murine nonalcoholic steatohepatitis.

Frontiers in immunology

Liao CY,Barrow F,Venkatesan N,Nakao Y,Mauer AS,Fredrickson G,Song MJ,Sehrawat TS,Dasgupta D,Graham RP,Revelo XS,Malhi H

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Tue May 09 00:00:00 EDT 2023

Alpha1-antitrypsin protects the immature mouse brain following hypoxic-ischemic injury.

Frontiers in cellular neuroscience

Zhang S,Li W,Xu Y,Li T,Ek J,Zhang X,Wang Y,Song J,Zhu C,Wang X

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Fri Mar 24 00:00:00 EDT 2023

Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation.

Cell reports

Gros M,Segura E,Rookhuizen DC,Baudon B,Heurtebise-Chrétien S,Burgdorf N,Maurin M,Kapp EA,Simpson RJ,Kozik P,Villadangos JA,Bertrand MJM,Burbage M,Amigorena S

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunocytochemistry

Tue Aug 16 00:00:00 EDT 2022

Phospholipid nanoparticles: Therapeutic potentials against atherosclerosis via reducing cholesterol crystals and inhibiting inflammation.

EBioMedicine

Luo Y,Guo Y,Wang H,Yu M,Hong K,Li D,Li R,Wen B,Hu D,Chang L,Zhang J,Yang B,Sun D,Schwendeman AS,Eugene Chen Y

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Wed Dec 01 00:00:00 EST 2021

KLF11 protects against abdominal aortic aneurysm through inhibition of endothelial cell dysfunction.

JCI insight

Zhao G,Chang Z,Zhao Y,Guo Y,Lu H,Liang W,Rom O,Wang H,Sun J,Zhu T,Fan Y,Chang L,Yang B,Garcia-Barrio MT,Chen YE,Zhang J

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunocytochemistry

Mon Mar 08 00:00:00 EST 2021

KLF11 protects against abdominal aortic aneurysm through inhibition of endothelial cell dysfunction.

JCI insight

Zhao G,Chang Z,Zhao Y,Guo Y,Lu H,Liang W,Rom O,Wang H,Sun J,Zhu T,Fan Y,Chang L,Yang B,Garcia-Barrio MT,Chen YE,Zhang J

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunocytochemistry

Mon Mar 08 00:00:00 EST 2021

Adult neural stem cells have latent inflammatory potential that is kept suppressed by Tcf4 to facilitate adult neurogenesis.

Science advances

Shariq M,Sahasrabuddhe V,Krishna S,Radha S,Bellampalli R,Dwivedi A,Cheramangalam R,Reizis B,Hébert J,Ghosh HS

50-5301-82 was used in Immunohistochemistry (PFA fixed) to provide evidence for a latent inflammatory potential of adult hippocampal neural progenitors and identify Tcf4 as a critical regulator that facilitates adult neurogenesis via proactive suppression of this detrimental potential.

Sat May 01 00:00:00 EDT 2021

Overexpression of Galectin 3 in Pancreatic β Cells Amplifies β-Cell Apoptosis and Islet Inflammation in Type-2 Diabetes in Mice.

Frontiers in endocrinology

Petrovic I,Pejnovic N,Ljujic B,Pavlovic S,Miletic Kovacevic M,Jeftic I,Djukic A,Draginic N,Andjic M,Arsenijevic N,Lukic ML,Jovicic N

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Wed Feb 03 00:00:00 EST 2021

Inhibition of p38mapk Reduces Adipose Tissue Inflammation in Aging Mediated by Arginase-II.

Pharmacology

Huang J,Liu C,Ming XF,Yang Z

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Wed Feb 24 00:00:00 EST 2021

Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis.

JCI insight

Lu S,Jolly AJ,Strand KA,Dubner AM,Mutryn MF,Moulton KS,Nemenoff RA,Majesky MW,Weiser-Evans MC

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Thu Dec 03 00:00:00 EST 2020

Macrophage NCOR1 Deficiency Ameliorates Myocardial Infarction and Neointimal Hyperplasia in Mice.

Journal of the American Heart Association

Du LJ,Sun JY,Zhang WC,Wang YL,Zhu H,Liu T,Gao MZ,Zheng C,Zhang YY,Liu Y,Liu Y,Shao S,Zhang XQ,Leng Q,Auwerx J,Duan SZ

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Tue Aug 04 00:00:00 EDT 2020

Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions.

Nature communications

O¿Shea TM,Wollenberg AL,Kim JH,Ao Y,Deming TJ,Sofroniew MV

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Fri Dec 04 00:00:00 EST 2020

Xanthohumol and 8-prenylnaringenin reduce type 2 diabetes-associated oxidative stress by downregulating galectin-3.

Porto biomedical journal

Luís C,Costa R,Rodrigues I,Castela Â,Coelho P,Guerreiro S,Gomes J,Reis C,Soares R

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Sun Apr 10 00:00:00 EDT 2022

Xanthohumol and 8-prenylnaringenin reduce type 2 diabetes-associated oxidative stress by downregulating galectin-3.

Porto biomedical journal

Luís C,Costa R,Rodrigues I,Castela Â,Coelho P,Guerreiro S,Gomes J,Reis C,Soares R

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Sun Apr 10 00:00:00 EDT 2022

Time-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation.

PloS one

Noguchi K,Tomita H,Kanayama T,Niwa A,Hatano Y,Hoshi M,Sugie S,Okada H,Niwa M,Hara A

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Fri Nov 01 00:00:00 EDT 2019

Myeloid-specific deletion of Zfp36 protects against insulin resistance and fatty liver in diet-induced obese mice.

American journal of physiology. Endocrinology and metabolism

Caracciolo V,Young J,Gonzales D,Ni Y,Flowers SJ,Summer R,Waldman SA,Kim JK,Jung DY,Noh HL,Kim T,Blackshear PJ,O'Connell D,Bauer RC,Kallen CB

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

T-Cell Mineralocorticoid Receptor Controls Blood Pressure by Regulating Interferon-Gamma.

Circulation research

Sun XN,Li C,Liu Y,Du LJ,Zeng MR,Zheng XJ,Zhang WC,Liu Y,Zhu M,Kong D,Zhou L,Lu L,Shen ZX,Yi Y,Du L,Qin M,Liu X,Hua Z,Sun S,Yin H,Zhou B,Yu Y,Zhang Z,Duan SZ

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Fri May 12 00:00:00 EDT 2017

SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer.

Journal of gastroenterology

Companioni Nápoles O,Tsao AC,Sanz-Anquela JM,Sala N,Bonet C,Pardo ML,Ding L,Simo O,Saqui-Salces M,Blanco VP,Gonzalez CA,Merchant JL

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Sun Jan 01 00:00:00 EST 2017

Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis.

JCI insight

Tomita K,Kohli R,MacLaurin BL,Hirsova P,Guo Q,Sanchez LHG,Gelbard HA,Blaxall BC,Ibrahim SH

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Thu Aug 03 00:00:00 EDT 2017

The kielin/chordin-like protein (KCP) attenuates high-fat diet-induced obesity and metabolic syndrome in mice.

The Journal of biological chemistry

Soofi A,Wolf KI,Emont MP,Qi N,Martinez-Santibanez G,Grimley E,Ostwani W,Dressler GR

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Fri Jun 02 00:00:00 EDT 2017

Galectin-3 expression in hippocampal CA2 following transient forebrain ischemia and its inhibition by hypothermia or antiapoptotic agents.

Neuroreport

Hisamatsu K,Niwa M,Kobayashi K,Miyazaki T,Hirata A,Hatano Y,Tomita H,Hara A

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunohistochemistry

Wed Mar 23 00:00:00 EDT 2016

Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.

Cell death & disease

Ilmer M,Mazurek N,Byrd JC,Ramirez K,Hafley M,Alt E,Vykoukal J,Bresalier RS

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Flow Cytometry

Thu Aug 11 00:00:00 EDT 2016

Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.

Cell death & disease

Ilmer M,Mazurek N,Byrd JC,Ramirez K,Hafley M,Alt E,Vykoukal J,Bresalier RS

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Flow Cytometry

Thu Aug 11 00:00:00 EDT 2016

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Imaging white adipose tissue with confocal microscopy.

Methods in enzymology

Martinez-Santibañez G,Cho KW,Lumeng CN

Published figure using Galectin 3 monoclonal antibody (Product # 50-5301-82) in Immunofluorescence

Fri Sep 19 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; MAC-2 antigen

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Gene Aliases: CBP35; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

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UniProt ID: (Human) Q96J47

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Entrez Gene ID: (Human) 3958, (Mouse) 16854

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Function(s)

protein binding IgE binding carbohydrate binding chemoattractant activity laminin binding poly(A) RNA binding Fc-gamma receptor I complex binding monosaccharide binding disaccharide binding advanced glycation end-product receptor activity extracellular matrix protein

Process(es)

monocyte chemotaxis mRNA processing RNA splicing neutrophil chemotaxis epithelial cell differentiation regulation of T cell proliferation innate immune response negative regulation of endocytosis eosinophil chemotaxis macrophage chemotaxis negative regulation of T cell receptor signaling pathway positive chemotaxis regulation of T cell apoptotic process mononuclear cell migration positive regulation of mononuclear cell migration positive regulation of calcium ion import regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of immunological synapse formation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway skeletal system development immune system process signal transduction positive regulation of cell proliferation positive regulation of serotonin secretion cell differentiation extracellular matrix organization negative regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell proliferation in bone marrow

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eFluor™ 660, eBioscience™

Galectin 3 Antibody (50-5301-82) in Flow

Product Details

50-5301-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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