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Gaussia luciferase Antibody (PA1-181) in WB
Western blot analysis of secreted Gaussia luciferase was performed using 293T cells stably expressing Gaussia or Cypridina luciferase. Cells were grown overnight in serum free (B27 supplemented) DMEM with or without 10uM Brefeldin A. Equal amounts (75 µg) of approximately 10x concentrated cell culture medium from treated or untreated cells, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) were loaded onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product #... View More
Product Details
PA1-181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Luciferase
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified recombinant Gaussia luciferase expressed in E. coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539912
Product Specific Information
Western blot analysis of PA1-181 detects a ~20 kDa protein in the supernatant of Gaussia luciferase-secreting cells.
PA1-181 can be used for activity-independent verification of Gaussia luciferase expression.
Target Information
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. Gaussia luciferase can be measured individually or in multiplex assays, such as the Gaussia-Firefly Luciferase Dual Assay Kit (Product # 16181). The Gaussia luciferase has been codon optimized for enhanced performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Gaussia luciferase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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