Invitrogen

GluR2 Monoclonal Antibody (6C4)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

33 References

View all (26) GluR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GluR2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

GluR2 Antibody (32-0300) in ICC/IF

Immunofluorescence analysis of GluR2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GluR2 (6C4) Mouse Monoclonal Antibody (Product # 32-0300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28... View More

GluR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GluR2 Antibody in Immunohistochemistry (IHC)

GluR2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

GluR2 Antibody — Figure 7 Lithium treatment significantly increases surface GluR1/GluR2 in the hippocampus, but not frontal cortex in vivo Mice were treated with lithium or control chow for 1.5 days. Following treatment, mice were decapitated and brains were rapidly removed. Hippocampal (HP) and frontal cortex (FC) tissues were isolated and the BS3 cross-linking procedure was performed to identify surface GluR1/GluR2 tetramers. Western blot analyses were performed with anti-GluR1 and anti-GluR2 antibodies. Representative images for cross-linked and intracellular GluR1 and GluR2 by western blot analysis (a, b). Densitometry analysis: Lithium significantly enhanced surface cross-linked GluR1 and GluR2 in hippocampus, but not in prefrontal cortex vivo. N=6 mice per group (b-e). * p <0.05 {TM}

Product Details

32-0300

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

View 7 publications 7 publications

ELISA (ELISA)

0.5-5 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Functional Assay (Functional)

- -

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6C4

Immunogen

Fusion protein containing amino acids 175-430 from the N-terminal region of GluR2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533058

Target Information

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Case report: Seropositive myasthenia gravis complicated by limbic encephalitis positive for antibodies to AMPAR and Lgi1.

Frontiers in neurology

Nes MS,Haugen M,Haugland HK,Gilhus NE,Vedeler CA

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunohistochemistry

Tue Oct 31 00:00:00 EDT 2023

Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.

Nature communications

Monsorno K,Ginggen K,Ivanov A,Buckinx A,Lalive AL,Tchenio A,Benson S,Vendrell M,D'Alessandro A,Beule D,Pellerin L,Mameli M,Paolicelli RC

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Sat Sep 16 00:00:00 EDT 2023

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission.

Science advances

Ma P,Wan LP,Li Y,He CH,Song NN,Zhao S,Wang H,Ding YQ,Mao B,Sheng N

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunocytochemistry

Fri Sep 30 00:00:00 EDT 2022

Role of Satb1 and Satb2 Transcription Factors in the Glutamate Receptors Expression and Ca²⁺ Signaling in the Cortical Neurons In Vitro.

International journal of molecular sciences

Turovsky EA,Turovskaya MV,Fedotova EI,Babaev AA,Tarabykin VS,Varlamova EG

32-0300 was used in Immunocytochemistry to demonstrate the participation of Satb1 and Satb2 transcription factors in NMDA-, AMPA-, and KA- receptor subunit gene expression.

Mon May 31 00:00:00 EDT 2021

Role of Satb1 and Satb2 Transcription Factors in the Glutamate Receptors Expression and Ca²⁺ Signaling in the Cortical Neurons In Vitro.

International journal of molecular sciences

Turovsky EA,Turovskaya MV,Fedotova EI,Babaev AA,Tarabykin VS,Varlamova EG

32-0300 was used in Immunocytochemistry to demonstrate the participation of Satb1 and Satb2 transcription factors in NMDA-, AMPA-, and KA- receptor subunit gene expression.

Mon May 31 00:00:00 EDT 2021

Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.

Science advances

Ban Y,Yu T,Feng B,Lorenz C,Wang X,Baker C,Zou Y

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Fri Oct 08 00:00:00 EDT 2021

Trans-synaptic assemblies link synaptic vesicles and neuroreceptors.

Science advances

Martinez-Sanchez A,Laugks U,Kochovski Z,Papantoniou C,Zinzula L,Baumeister W,Lučić V

32-0300 was used in Sample Preparation to investigate if synaptic function is carried by precisely organised trans-synaptic units.

Mon Mar 01 00:00:00 EST 2021

Evidence that Brain-Reactive Autoantibodies Contribute to Chronic Neuronal Internalization of Exogenous Amyloid-β1-42 and Key Cell Surface Proteins During Alzheimer's Disease Pathogenesis.

Journal of Alzheimer's disease : JAD

Goldwaser EL,Acharya NK,Wu H,Godsey GA,Sarkar A,DeMarshall CA,Kosciuk MC,Nagele RG

32-0300 was used in Immunohistochemistry to examine consequences of increased blood-brain barrier (BBB) permeability on the development of Alzheimer's disease (AD)-related pathology by tracking selected leaked plasma components and their interactions with neurons in vivo and in vitro.

Thu May 13 00:00:00 EDT 2021

Distinct Cell Surface Expression Patterns of N-Glycosylation Site Mutants of AMPA-Type Glutamate Receptor under the Homo-Oligomeric Expression Conditions.

International journal of molecular sciences

Morise J,Yamamoto S,Midorikawa R,Takamiya K,Nonaka M,Takematsu H,Oka S

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunocytochemistry

Sun Jul 19 00:00:00 EDT 2020

Distinct Cell Surface Expression Patterns of N-Glycosylation Site Mutants of AMPA-Type Glutamate Receptor under the Homo-Oligomeric Expression Conditions.

International journal of molecular sciences

Morise J,Yamamoto S,Midorikawa R,Takamiya K,Nonaka M,Takematsu H,Oka S

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunocytochemistry

Sun Jul 19 00:00:00 EDT 2020

Sex differences in the role of atypical PKC within the basolateral nucleus of the amygdala in a mouse hyperalgesic priming model.

Neurobiology of pain (Cambridge, Mass.)

Baptista-de-Souza D,Tavares-Ferreira D,Megat S,Sankaranarayanan I,Shiers S,Flores CM,Ghosh S,Luiz Nunes-de-Souza R,Canto-de-Souza A,Price TJ

32-0300 was used in Immunohistochemistry to elucidate the molecular mechanisms in the basolateral nucleus of the amygdala (BLA) that modulate hyperalgesic priming, a pain plasticity model, in males and females.

Mon Sep 28 00:00:00 EDT 2020

Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.

Cell reports

Pensalfini A,Kim S,Subbanna S,Bleiwas C,Goulbourne CN,Stavrides PH,Jiang Y,Lee JH,Darji S,Pawlik M,Huo C,Peddy J,Berg MJ,Smiley JF,Basavarajappa BS,Nixon RA

32-0300 was used in Immunocytochemistry to report that Rab5 overactivation alone recapitulates diverse prodromal and degenerative features of AD.

Tue Nov 24 00:00:00 EST 2020

Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.

Cell reports

Pensalfini A,Kim S,Subbanna S,Bleiwas C,Goulbourne CN,Stavrides PH,Jiang Y,Lee JH,Darji S,Pawlik M,Huo C,Peddy J,Berg MJ,Smiley JF,Basavarajappa BS,Nixon RA

32-0300 was used in Immunocytochemistry to report that Rab5 overactivation alone recapitulates diverse prodromal and degenerative features of AD.

Tue Nov 24 00:00:00 EST 2020

Glutamate Receptor Trafficking and Protein Synthesis Mediate the Facilitation of LTP by Secreted Amyloid Precursor Protein-Alpha.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mockett BG,Guévremont D,Elder MK,Parfitt KD,Peppercorn K,Morrissey J,Singh A,Hintz TJ,Kochen L,Tom Dieck S,Schuman E,Tate WP,Williams JM,Abraham WC

32-0300 was used in Western Blotting to demonstrate that exposure to secreted amyloid precursor protein-alpha converts short-lasting long-term potentiation into protein-synthesis-dependent late LTP in hippocampal slices from male rats.

Wed Apr 24 00:00:00 EDT 2019

The Temporal Dynamics of Arc Expression Regulate Cognitive Flexibility.

Neuron

Wall MJ,Collins DR,Chery SL,Allen ZD,Pastuzyn ED,George AJ,Nikolova VD,Moy SS,Philpot BD,Shepherd JD,Müller J,Ehlers MD,Mabb AM,Corrêa SAL

32-0300 was used in Western Blotting to study how the precise temporal control of activity-dependent molecules, such as Arc, regulates synaptic plasticity and is crucial for cognition.

Wed Jun 27 00:00:00 EDT 2018

Mice with Shank3 Mutations Associated with ASD and Schizophrenia Display Both Shared and Distinct Defects.

Neuron

Zhou Y,Kaiser T,Monteiro P,Zhang X,Van der Goes MS,Wang D,Barak B,Zeng M,Li C,Lu C,Wells M,Amaya A,Nguyen S,Lewis M,Sanjana N,Zhou Y,Zhang M,Zhang F,Fu Z,Feng G

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Wed Jan 06 00:00:00 EST 2016

NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.

Nature communications

Frank RA,Komiyama NH,Ryan TJ,Zhu F,O'Dell TJ,Grant SG

32-0300 was used in western blot to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors

Wed Apr 27 00:00:00 EDT 2016

Cocaine-induced plasticity in the cerebellum of sensitised mice.

Psychopharmacology

Vazquez-Sanroman D,Carbo-Gas M,Leto K,Cerezo-Garcia M,Gil-Miravet I,Sanchis-Segura C,Carulli D,Rossi F,Miquel M

32-0300 was used in immunohistochemistry to study sensitized mice and cocaine-induced plasticity in the cerebellum

Tue Dec 01 00:00:00 EST 2015

L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway.

Cell death & disease

Hao JR,Sun N,Lei L,Li XY,Yao B,Sun K,Hu R,Zhang X,Shi XD,Gao C

32-0300 was used in western blot to utilize models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway where L-Stepholidine rescues memory deficit and synaptic plasticity

Thu Nov 05 00:00:00 EST 2015

Rapid neurogenesis through transcriptional activation in human stem cells.

Molecular systems biology

Busskamp V,Lewis NE,Guye P,Ng AH,Shipman SL,Byrne SM,Sanjana NE,Murn J,Li Y,Li S,Stadler M,Weiss R,Church GM

32-0300 was used in immunocytochemistry to describe a method to rapidly generate neurons from human-induced pluripotent stem cells to elucidate the underlying regulatory programs

Mon Nov 17 00:00:00 EST 2014

GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons.

Molecular and cellular neurosciences

Ho VM,Dallalzadeh LO,Karathanasis N,Keles MF,Vangala S,Grogan T,Poirazi P,Martin KC

32-0300 was used in western blot to test if synaptic GluA2 concentrations are regulated protein trafficking or local translation.

Tue Jul 01 00:00:00 EDT 2014

GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons.

Molecular and cellular neurosciences

Ho VM,Dallalzadeh LO,Karathanasis N,Keles MF,Vangala S,Grogan T,Poirazi P,Martin KC

32-0300 was used in western blot to test if synaptic GluA2 concentrations are regulated protein trafficking or local translation.

Tue Jul 01 00:00:00 EDT 2014

GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons.

Molecular and cellular neurosciences

Ho VM,Dallalzadeh LO,Karathanasis N,Keles MF,Vangala S,Grogan T,Poirazi P,Martin KC

32-0300 was used in western blot to test if synaptic GluA2 concentrations are regulated protein trafficking or local translation.

Tue Jul 01 00:00:00 EDT 2014

Redistribution of ionotropic glutamate receptors detected by laser microdissection of the rat dentate gyrus 48 h following LTP induction in vivo.

PloS one

Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM

32-0300 was used in western blot to study the effect of LTP induction on the distribution of ionotropic glutamate receptors in vivo

Fri Jan 30 00:00:00 EST 2015

Rapid visual stimulation increases extrasynaptic glutamate receptor expression but not visual-evoked potentials in the adult rat primary visual cortex.

The European journal of neuroscience

Eckert MJ,Guévremont D,Williams JM,Abraham WC

32-0300 was used in western blot to study the visual-evoked potential in the primary visual cortex of adult rats.

Fri Feb 01 00:00:00 EST 2013

Increased expression, but not postsynaptic localisation, of ionotropic glutamate receptors during the late-phase of long-term potentiation in the dentate gyrus in vivo.

Neuropharmacology

Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM

Thu Jan 01 00:00:00 EST 2009

Involvement of AMPA receptors in the antidepressant-like effects of lithium in the mouse tail suspension test and forced swim test.

Neuropharmacology

Gould TD,O'Donnell KC,Dow ER,Du J,Chen G,Manji HK

32-0300 was used in western blot to elucidate how lithium exerts its antidepressant effects

Sat Mar 01 00:00:00 EST 2008

Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC

Wed Dec 19 00:00:00 EST 2007

Learning-induced glutamate receptor phosphorylation resembles that induced by long term potentiation.

The Journal of biological chemistry

Shukla K,Kim J,Blundell J,Powell CM

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunoprecipitation

Fri Jun 22 00:00:00 EDT 2007

Learning-induced glutamate receptor phosphorylation resembles that induced by long term potentiation.

The Journal of biological chemistry

Shukla K,Kim J,Blundell J,Powell CM

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunoprecipitation

Fri Jun 22 00:00:00 EDT 2007

A molecular mechanism for stabilization of learning-induced synaptic modifications.

Neuron

Quinlan EM,Lebel D,Brosh I,Barkai E

Thu Jan 22 00:00:00 EST 2004

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 EDT 2003

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 EDT 2003

Glutamate receptor subunit GluR2 and NMDAR1 immunoreactivity in the retina of macaque monkeys with experimental glaucoma does not identify vulnerable neurons.

Experimental neurology

Hof PR,Lee PY,Yeung G,Wang RF,Podos SM,Morrison JH

Thu Oct 01 00:00:00 EDT 1998

Structural determinants of ion flow through recombinant glutamate receptor channels.

Science (New York, N.Y.)

Verdoorn TA,Burnashev N,Monyer H,Seeburg PH,Sakmann B

Fri Jun 21 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: AMPA selective glutamate receptor; AMPA-selective glutamate receptor 2; GluR-2; GluR-B; GluR-K2; glutamate receptor; Glutamate receptor 2; glutamate receptor B; glutamate receptor B flip isoform; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, AMPA 2; OTTHUMP00000165324; OTTHUMP00000220942; OTTHUMP00000220943

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Gene Aliases: GluA2; Glur-2; GluR-B; GluR-K2; GLUR2; GLURB; GRIA2; HBGR2

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UniProt ID: (Human) P42262, (Rat) P19491

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Entrez Gene ID: (Human) 2891, (Mouse) 14800, (Rat) 29627

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Function(s)

ionotropic glutamate receptor activity AMPA glutamate receptor activity excitatory extracellular ligand-gated ion channel activity extracellular-glutamate-gated ion channel activity protein binding receptor activity ion channel activity kainate selective glutamate receptor activity protein kinase binding PDZ domain binding identical protein binding calcium channel regulator activity transmembrane signal receptor

Process(es)

signal transduction chemical synaptic transmission ion transmembrane transport ionotropic glutamate receptor signaling pathway regulation of receptor recycling transport ion transport receptor internalization synaptic transmission, glutamatergic establishment of protein localization positive regulation of synaptic transmission protein tetramerization regulation of synaptic transmission, glutamatergic response to lithium ion response to fungicide

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GluR2 Monoclonal Antibody (6C4)

GluR2 Antibody (32-0300) in ICC/IF

Product Details

32-0300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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