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Glucagon Antibody (710316) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Panc-1 (Lane 1) and 10 µL of Panc-1 serum starved (ss) conditioned media (serum starved for overnight) (Lane 2).The blots were probed with Glucagon Recombinant Rabbit Polyclonal Antibody (Product # 710316, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 25 kDa band corresponding to Glucagon was observed across the cell line tested. K... View More
Product Details
710316
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
3HCLC
Immunogen
Peptide corresponding to amino acids 53-81 of human Glucagon
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532683
Product Specific Information
This antibody is predicted to react with mouse, rat, rabbit and porcine based on sequence homology.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glicentin-related polypeptide; GLP1/2; glucagon-like peptide 1; glucagon-like peptide 2; IL12p35; IL12P40; OXY; preproglucagon; Pro-glucagon
Gene Aliases: GCG; GLP1; GLP2; GRPP
UniProt ID: (Human) P01275
Entrez Gene ID: (Human) 2641
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
feeding behavior
cell proliferation
protein kinase A signaling
positive regulation of peptidyl-threonine phosphorylation
positive regulation of cAMP biosynthetic process
positive regulation of protein binding
negative regulation of appetite
positive regulation of peptidyl-serine phosphorylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
response to starvation
negative regulation of apoptotic process
positive regulation of protein kinase activity
regulation of insulin secretion
positive regulation of histone H3-K4 methylation
positive regulation of ERK1 and ERK2 cascade
cellular response to glucagon stimulus
positive regulation of calcium ion import
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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