Invitrogen

Glucocorticoid Receptor Polyclonal Antibody

Glucocorticoid Receptor Antibody in Immunocytochemistry (ICC/IF)

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Glucocorticoid Receptor Antibody (PA1-510A) in ICC/IF

Immunofluorescence analysis of Glucocorticoid Receptor was performed using 70% confluent log phase A-549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Glucocorticoid Receptor Rabbit Polyclonal Antibody (Product # PA1-510A) at 1:250 dilution in 0.1% ... View More

Glucocorticoid Receptor Antibody in Immunohistochemistry (IHC)

Glucocorticoid Receptor Antibody in Western Blot (WB)

Glucocorticoid Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Glucocorticoid Receptor Antibody in Gel Shift (GS)

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Product Details

PA1-510A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,500

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:250

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

View 4 publications 4 publications

Gel Shift (GS)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Reptile, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: A(150) P T E K E F P K T H S D V S S E Q Q H L K G Q T G(175)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325823

Product Specific Information

PA1-510A detects glucocorticoid receptor (GR) from human, rat, and reptile tissues. This product detects both the unactivated and activated forms of the receptor.

PA1-510A has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract.

PA1-510A immunizing peptide corresponds to amino acid residues 150- 175 from human GR.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of global inhibitors of cellular glycosylation.

Nature communications

Sørensen DM,Büll C,Madsen TD,Lira-Navarrete E,Clausen TM,Clark AE,Garretson AF,Karlsson R,Pijnenborg JFA,Yin X,Miller RL,Chanda SK,Boltje TJ,Schjoldager KT,Vakhrushev SY,Halim A,Esko JD,Carlin AF,Hurtado-Guerrero R,Weigert R,Clausen H,Narimatsu Y

PA1-510A was used in Immunocytochemistry-immunofluorescence to demonstrate how these inhibitors can be used to manipulate glycosylation in cells to induce expression of truncated O-glycans and augment binding of cancer-specific Tn-glycoprotein antibodies and to inhibit expression of heparan sulfate and binding and infection of SARS-CoV-2.

Mon Feb 20 00:00:00 EST 2023

Genome-Wide Identification of Basic Helix-Loop-Helix and NF-1 Motifs Underlying GR Binding Sites in Male Rat Hippocampus.

Endocrinology

Pooley JR,Flynn BP,Grøntved L,Baek S,Guertin MJ,Kershaw YM,Birnie MT,Pellatt A,Rivers CA,Schiltz RL,Hager GL,Lightman SL,Conway-Campbell BL

PA1-510A was used in Chromatin immunoprecipitation to study the structure and function of glucocorticoid receptors in the hippocampus.

Mon May 01 00:00:00 EDT 2017

Reprogramming the chromatin landscape: interplay of the estrogen and glucocorticoid receptors at the genomic level.

Cancer research

Miranda TB,Voss TC,Sung MH,Baek S,John S,Hawkins M,Grøntved L,Schiltz RL,Hager GL

PA1-510A was used in ChIP assay to study the role of chromatin reorganization in the genomic level interactions between the estrogen and glucocorticoid receptors

Thu Aug 15 00:00:00 EDT 2013

Enhanced stimulus sequence-dependent repeated learning in male offspring after prenatal stress alone or in conjunction with lead exposure.

Neurotoxicology

Cory-Slechta DA,Virgolini MB,Liu S,Weston D

PA1-510A was used in western blot to study the effects of prenatal stress alone or in conjunction with lead exposure on learning in male offspring

Mon Oct 01 00:00:00 EDT 2012

Decreased levels of nuclear glucocorticoid receptor protein in the hippocampus of aged Long-Evans rats with cognitive impairment.

Brain research

Lee SY,Hwang YK,Yun HS,Han JS

PA1-510A was used in EMSA to study hippocampal GR signaling in cognitively impaired aged rats

Wed Oct 10 00:00:00 EDT 2012

Transcription factor AP1 potentiates chromatin accessibility and glucocorticoid receptor binding.

Molecular cell

Biddie SC,John S,Sabo PJ,Thurman RE,Johnson TA,Schiltz RL,Miranda TB,Sung MH,Trump S,Lightman SL,Vinson C,Stamatoyannopoulos JA,Hager GL

PA1-510A was used in ChIP assay to investigate the importance of AP1 in GR-mediated transcriptional regulation

Fri Jul 08 00:00:00 EDT 2011

Distribution and subcellular localization of glucocorticoid receptor-immunoreactive neurons in the developing and adult male zebra finch brain.

General and comparative endocrinology

Shahbazi M,Schmidt M,Carruth LL

PA1-510A was used in immunohistochemistry to characterize the glucocorticoid receptor-immunoreactive neurons in male zebra finch brain

Thu Dec 01 00:00:00 EST 2011

Intratympanic dexamethasone as initial therapy for idiopathic sudden sensorineural hearing loss: Clinical evaluation and laboratory investigation.

Auris, nasus, larynx

Fu Y,Zhao H,Zhang T,Chi F

PA1-510A was used in immunohistochemistry to investigate the therapeutic efficacy of intratympanic dexamethasone for idiopathic sudden sensorineural hearing loss

Fri Apr 01 00:00:00 EDT 2011

Administration of glucocorticoids to ovarian cancer patients is associated with expression of the anti-apoptotic genes SGK1 and MKP1/DUSP1 in ovarian tissues.

Clinical cancer research : an official journal of the American Association for Cancer Research

Melhem A,Yamada SD,Fleming GF,Delgado B,Brickley DR,Wu W,Kocherginsky M,Conzen SD

PA1-510A was used in western blot to study the effect of glucocorticoid on SGK1 and MKP1 expression in ovarian tumors.

Fri May 01 00:00:00 EDT 2009

Administration of glucocorticoids to ovarian cancer patients is associated with expression of the anti-apoptotic genes SGK1 and MKP1/DUSP1 in ovarian tissues.

Clinical cancer research : an official journal of the American Association for Cancer Research

Melhem A,Yamada SD,Fleming GF,Delgado B,Brickley DR,Wu W,Kocherginsky M,Conzen SD

PA1-510A was used in western blot to study the effect of glucocorticoid on SGK1 and MKP1 expression in ovarian tumors.

Fri May 01 00:00:00 EDT 2009

Characterization of the angiotensin (AT1b) receptor promoter and its regulation by glucocorticoids.

Journal of molecular endocrinology

Bogdarina IG,King PJ,Clark AJ

PA1-510A was used in EMSA to investigate the angiotensin receptor promoter and study the effect of glucocorticoids on its activity.

Sat Aug 01 00:00:00 EDT 2009

Changes of glucocorticoid receptor expression in the nasal polyps of patients with chronic sinusitis following treatment with glucocorticoid.

In vivo (Athens, Greece)

Watanabe S,Suzaki H

PA1-510A was used in immunohistochemistry to investigate the level of glucocorticoid receptor protein in chronic sinusitis patients treated with glucocorticoids

Tue May 06 00:00:00 EDT 2008

Involvement of {beta}-catenin and unusual behavior of CBP and p300 in glucocorticosteroid signaling in Schwann cells.

Proceedings of the National Academy of Sciences of the United States of America

Fonte C,Grenier J,Trousson A,Chauchereau A,Lahuna O,Baulieu EE,Schumacher M,Massaad C

PA1-510A was used in immunocytochemistry to investigate the roles of CREB-binding protein and p300 during the glucocorticosteroid signaling.

Tue Oct 04 00:00:00 EDT 2005

Effects of glucocorticoids on the growth and chemosensitivity of carcinoma cells are heterogeneous and require high concentration of functional glucocorticoid receptors.

World journal of gastroenterology

Lu YS,Lien HC,Yeh PY,Yeh KH,Kuo ML,Kuo SH,Cheng AL

Published figure using Glucocorticoid Receptor polyclonal antibody (Product # PA1-510A) in Immunohistochemistry (Paraffin)

Fri Oct 28 00:00:00 EDT 2005

Anthrax lethal toxin represses glucocorticoid receptor (GR) transactivation by inhibiting GR-DNA binding in vivo.

Molecular and cellular endocrinology

Webster JI,Sternberg EM

PA1-510A was used in immunohistochemistry to investigate the mechanism for the effect of anthrax lethal toxin on glucocorticoid receptor transactivation

Wed Sep 28 00:00:00 EDT 2005

Interactions of chronic lead exposure and intermittent stress: consequences for brain catecholamine systems and associated behaviors and HPA axis function.

Toxicological sciences : an official journal of the Society of Toxicology

Virgolini MB,Chen K,Weston DD,Bauter MR,Cory-Slechta DA

PA1-510A was used in western blot to investigate the effect of lead exposure and intermittent stress on brain catecholamine systems

Sat Oct 01 00:00:00 EDT 2005

Intronic hormone response elements mediate regulation of FKBP5 by progestins and glucocorticoids.

Cell stress & chaperones

Hubler TR,Scammell JG

PA1-510A was used in EMSA assay to study how FKBP5 is regulated by glucocorticoids and progestins through distal intronic HREs

Mon Apr 25 00:00:00 EDT 2005

Selective recruitment of p160 coactivators on glucocorticoid-regulated promoters in Schwann cells.

Molecular endocrinology (Baltimore, Md.)

Grenier J,Trousson A,Chauchereau A,Amazit L,Lamirand A,Leclerc P,Guiochon-Mantel A,Schumacher M,Massaad C

PA1-510A was used in immunocytochemistry to investigate the interaction between steroid receptor coactivator 1 and glucocorticoid receptor in Schwann cells

Wed Dec 01 00:00:00 EST 2004

Transcriptional inhibition of the human insulin receptor gene by aldosterone.

The Journal of steroid biochemistry and molecular biology

Calle C,Campión J,García-Arencibia M,Maestro B,Dávila N

PA1-510A was used in EMSA to investigate the effect of aldosterone on the transcription of human insulin receptor gene

Tue Apr 01 00:00:00 EST 2003

CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins.

Molecular endocrinology (Baltimore, Md.)

Lambert JR,Nordeen SK

PA1-510A was used in ChIP assay to investigate the role of glucocorticoids and progestins for CBP recruitment and histone acetylation in differential gene induction

Sun Jun 01 00:00:00 EDT 2003

Identification of a novel dexamethasone responsive enhancer in the human CYP3A5 gene and its activation in human and rat liver cells.

Molecular pharmacology

Schuetz JD,Schuetz EG,Thottassery JV,Guzelian PS,Strom S,Sun D

PA1-510A was used in EMSA assay to study how the human CYP3A5 gene is regulated by a novel dexamethasone responsive enhancer

Mon Jan 01 00:00:00 EST 1996

Mutation of isoleucine 747 by a threonine alters the ligand responsiveness of the human glucocorticoid receptor.

Molecular endocrinology (Baltimore, Md.)

Roux S,Térouanne B,Balaguer P,Jausons-Loffreda N,Pons M,Chambon P,Gronemeyer H,Nicolas JC

PA1-510A was used in western blot to perform mutational analysis on human glucocorticoid receptor

Tue Oct 01 00:00:00 EDT 1996

Bioinformatics

Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858

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Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1

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UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536

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Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding glucocorticoid receptor activity steroid binding protein binding zinc ion binding protein complex binding glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity steroid hormone binding RNA polymerase II regulatory region DNA binding DNA binding chromatin binding double-stranded DNA binding steroid hormone receptor activity transcription coactivator activity transcription factor binding lipid binding Hsp70 protein binding receptor tyrosine kinase binding heat shock protein binding hormone binding protein homodimerization activity sequence-specific DNA binding metal ion binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding

Process(es)

regulation of gluconeogenesis chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glucocorticoid metabolic process regulation of glucose metabolic process positive regulation of glutamate secretion chromatin modification adrenal gland development negative regulation of synaptic plasticity regulation of glucocorticoid biosynthetic process regulation of cell proliferation maternal behavior glucocorticoid receptor signaling pathway negative regulation of vascular permeability steroid hormone mediated signaling pathway glucocorticoid mediated signaling pathway positive regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter response to arsenic-containing substance chromatin-mediated maintenance of transcription mammary gland duct morphogenesis positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to steroid hormone stimulus cellular response to dexamethasone stimulus negative regulation of glucocorticoid mediated signaling pathway positive regulation of glucocorticoid receptor signaling pathway brain development aging circadian rhythm androgen metabolic process response to radiation response to activity response to inactivity muscle atrophy lung development response to insulin response to mercury ion response to corticosterone response to calcium ion response to electrical stimulus cellular response to magnesium ion response to dexamethasone transcription initiation from RNA polymerase II promoter apoptotic process chromosome segregation mitotic nuclear division signal transduction covalent chromatin modification cell division

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Glucocorticoid Receptor Polyclonal Antibody

Glucocorticoid Receptor Antibody (PA1-510A) in ICC/IF

Product Details

PA1-510A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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