Invitrogen

Glucocorticoid Receptor Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

31 References

View all (67) Glucocorticoid Receptor (Nr3c1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Glucocorticoid Receptor Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Glucocorticoid Receptor Antibody (PA1-512) in ICC/IF

Immunofluorescence analysis of Glucocorticoid Receptor was performed using MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 µM Dexamethasone treatment for 2 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled ... View More

Glucocorticoid Receptor Antibody in Western Blot (WB)

Glucocorticoid Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP)

Glucocorticoid Receptor Antibody in ChIP Assay (ChIP)

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Product Details

PA1-512

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

Immunocytochemistry (ICC/IF)

1:20-1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

5 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-dependent

View 3 publications 3 publications

Gel Shift (GS)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2283110

Product Specific Information

PA1-512 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.

PA1-512 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, ChIP and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. Immunohistochemical staining of GR in rat hippocampus using PA1-512 results in staining primarily in the nucleus. PA1-512 does not disrupt receptor-DNA interactions.

The PA1-512 immunogen is a synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR. The PA1-512 immunizing peptide (Cat. # PEP-002) is available for use in for neutralization and control experiments.

Reconstitute in 50 µL PBS to create a stock of 1 mg/mL.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sensitivity and Resilience to Predator Stress-Enhanced Ethanol Drinking Is Associated With Sex-Dependent Differences in Stress-Regulating Systems.

Frontiers in behavioral neuroscience

Alavi M,Ryabinin AE,Helms ML,Nipper MA,Devaud LL,Finn DA

PA1-512 was used in Western Blotting to characterize sensitivity" and "resilience" to predator stress-enhanced drinking."

Fri Nov 04 00:00:00 EDT 2022

GLCCI1 is a novel protector against glucocorticoid-induced apoptosis in T cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kiuchi Z,Nishibori Y,Kutsuna S,Kotani M,Hada I,Kimura T,Fukutomi T,Fukuhara D,Ito-Nitta N,Kudo A,Takata T,Ishigaki Y,Tomosugi N,Tanaka H,Matsushima S,Ogasawara S,Hirayama Y,Takematsu H,Yan K

PA1-512 was used in Western Blot to show that transgenic mice overexpressing human GLCCI1 displayed enlarged thymi that consisted of larger numbers of thymocytes.

Sat Jun 01 00:00:00 EDT 2019

Corticosterone administration targeting a hypo-reactive HPA axis rescues a socially-avoidant phenotype in scarcity-adversity reared rats.

Developmental cognitive neuroscience

Perry RE,Rincón-Cortés M,Braren SH,Brandes-Aitken AN,Opendak M,Pollonini G,Chopra D,Raver CC,Alberini CM,Blair C,Sullivan RM

PA1-512 was used in Western Blotting to provide causal support that early-life scarcity-adversity exposure negatively impacts social development via a hypocorticosteronism-dependent mechanism, which can be targeted via CORT administration to rescue social behavior.

Sun Dec 01 00:00:00 EST 2019

Tumor suppressor protein (p)53, is a regulator of NF-kappaB repression by the glucocorticoid receptor.

Proceedings of the National Academy of Sciences of the United States of America

Murphy SH,Suzuki K,Downes M,Welch GL,De Jesus P,Miraglia LJ,Orth AP,Chanda SK,Evans RM,Verma IM

PA1-512 was used in immunocytochemistry to investigate the effect of p53 on NF-kappaB repression by the glucocorticoid receptor

Tue Oct 11 00:00:00 EDT 2011

Cross talk between glucocorticoid and estrogen receptors occurs at a subset of proinflammatory genes.

Journal of immunology (Baltimore, Md. : 1950)

Cvoro A,Yuan C,Paruthiyil S,Miller OH,Yamamoto KR,Leitman DC

PA1-512 was used in western blot to study cross talk between glucocorticoid and estrogen receptors at a subset of proinflammatory genes

Fri Apr 01 00:00:00 EDT 2011

Progesterone receptor directly inhibits β-casein gene transcription in mammary epithelial cells through promoting promoter and enhancer repressive chromatin modifications.

Molecular endocrinology (Baltimore, Md.)

Buser AC,Obr AE,Kabotyanski EB,Grimm SL,Rosen JM,Edwards DP

PA1-512 was used in ChIP assay to study the effect of progesterone receptor on casein gene transcription in mammary epithelial cells and its mechanism

Wed Jun 01 00:00:00 EDT 2011

Enhanced neutrophil expression of annexin-1 in coronary artery disease.

Metabolism: clinical and experimental

Särndahl E,Bergström I,Nijm J,Forslund T,Perretti M,Jonasson L

PA1-512 was used in flow cytometry to investigate the expression of annexin-1 in neutrophils in coronary artery disease patients

Mon Mar 01 00:00:00 EST 2010

Stealth-nanoparticle strategy for enhancing the efficacy of steroids in mice with noise-induced hearing loss.

Nanomedicine (London, England)

Horie RT,Sakamoto T,Nakagawa T,Ishihara T,Higaki M,Ito J

PA1-512 was used in immunohistochemistry to evaluate the therapeutic effect of nano-particles for steroid delivery in mice

Mon Nov 01 00:00:00 EDT 2010

ATP hydrolysis is essential for Bag-1M-mediated inhibition of the DNA binding by the glucocorticoid receptor.

Biochemical and biophysical research communications

Hong W,Chen L,Liu Y,Gao W

PA1-512 was used in EMSA to study the involvement of ATP hydrolysis in the interplay of Bag-1M and glucocorticoid receptor

Fri Dec 04 00:00:00 EST 2009

Bag-1M is a component of the in vivo DNA-glucocorticoid receptor complex at hormone-regulated promoter.

Journal of molecular biology

Hong W,Baniahmad A,Liu Y,Li H

PA1-512 was used in western blot to investigate the influence of Bag-1M on gene expression modulated by glucocorticoid receptor

Fri Dec 05 00:00:00 EST 2008

The effect of long-term exposure to combinations of growth promoters in Long Evans rats: part 2. Adrenal morphology (histopathology and immunochemical studies).

Analytica chimica acta

Illera JC,Peña L,Martínez-Mateos MM,Camacho L,Blass A,Garcia-Partida P,Illera MJ,Silván G

PA1-512 was used in immunohistochemistry to investigate the effect of growth promoters on adrenal morphology and glucocorticoid receptor expression

Wed Mar 14 00:00:00 EDT 2007

A reduction state potentiates the glucocorticoid response through receptor protein stabilization.

Genes to cells : devoted to molecular & cellular mechanisms

Kitagawa H,Yamaoka I,Akimoto C,Kase I,Mezaki Y,Shimizu T,Kato S

Published figure using Glucocorticoid Receptor polyclonal antibody (Product # PA1-512) in Immunoprecipitation

Thu Nov 01 00:00:00 EDT 2007

A reduction state potentiates the glucocorticoid response through receptor protein stabilization.

Genes to cells : devoted to molecular & cellular mechanisms

Kitagawa H,Yamaoka I,Akimoto C,Kase I,Mezaki Y,Shimizu T,Kato S

Published figure using Glucocorticoid Receptor polyclonal antibody (Product # PA1-512) in Immunoprecipitation

Thu Nov 01 00:00:00 EDT 2007

Transcriptional regulation of the mouse IL-7 receptor alpha promoter by glucocorticoid receptor.

Journal of immunology (Baltimore, Md. : 1950)

Lee HC,Shibata H,Ogawa S,Maki K,Ikuta K

PA1-512 was used in chromatin immunoprecipitation to investigate the role of the glucocorticoid receptor during the transcriptional regulation of the mouse IL-7 receptor alpha promoter.

Wed Jun 15 00:00:00 EDT 2005

STAT3-dependent enhanceosome assembly and disassembly: synergy with GR for full transcriptional increase of the alpha 2-macroglobulin gene.

Genes & development

Lerner L,Henriksen MA,Zhang X,Darnell JE

PA1-512 was used in chromatin immunoprecipitation to investigate the role of GR during the STAT3-dependent enhanceosome assembly and disassembly.

Wed Oct 15 00:00:00 EDT 2003

Identification of a novel glucocorticoid receptor mutation in budesonide-resistant human bronchial epithelial cells.

Molecular endocrinology (Baltimore, Md.)

Kunz S,Sandoval R,Carlsson P,Carlstedt-Duke J,Bloom JW,Miesfeld RL

PA1-512 was used in western blot to identify the mutation of a novel glucocorticoid receptor in budesonide-resistant human bronchial epithelial cells.

Mon Dec 01 00:00:00 EST 2003

ATP-dependent mobilization of the glucocorticoid receptor during chromatin remodeling.

Molecular and cellular biology

Fletcher TM,Xiao N,Mautino G,Baumann CT,Wolford R,Warren BS,Hager GL

PA1-512 was used in western blot to investigate the mobilization movement of glucocorticoid receptor during chromatin remodeling process.

Wed May 01 00:00:00 EDT 2002

Functional modulation of the glucocorticoid receptor and suppression of NF-kappaB-dependent transcription by ursodeoxycholic acid.

The Journal of biological chemistry

Miura T,Ouchida R,Yoshikawa N,Okamoto K,Makino Y,Nakamura T,Morimoto C,Makino I,Tanaka H

PA1-512 was used in immunocytochemistry to study the molecular mechanism of UDCA action.

Fri Dec 14 00:00:00 EST 2001

Beta(2)-adrenergic receptors potentiate glucocorticoid receptor transactivation via G protein beta gamma-subunits and the phosphoinositide 3-kinase pathway.

Molecular endocrinology (Baltimore, Md.)

Schmidt P,Holsboer F,Spengler D

PA1-512 was used in western blot to study the effect of beta(2)-adrenergic receptors on glucocorticoid receptor transactivation and its mechanism.

Sun Apr 01 00:00:00 EST 2001

Glucocorticoid resistance in the squirrel monkey is associated with overexpression of the immunophilin FKBP51.

The Journal of clinical endocrinology and metabolism

Reynolds PD,Ruan Y,Smith DF,Scammell JG

PA1-512 was used in western blot to study the expression of the hsp90-associated immunophilin FKBP51 in squirrel monkeys and its effect on glucocorticoid receptor.

Mon Feb 01 00:00:00 EST 1999

Pharmacological characterization of glucocorticoid receptors in primary human bronchial epithelial cells.

Biochemical pharmacology

LeVan TD,Babin EA,Yamamura HI,Bloom JW

PA1-512 was used in western blot to investigate the expression and activity of glucocorticoid receptors in primary bronchial epithelial cells

Sat May 01 00:00:00 EDT 1999

Redox-dependent regulation of nuclear import of the glucocorticoid receptor.

The Journal of biological chemistry

Okamoto K,Tanaka H,Ogawa H,Makino Y,Eguchi H,Hayashi S,Yoshikawa N,Poellinger L,Umesono K,Makino I

PA1-512 was used in immunohistochemistry to investigate the role of the redox during the nuclear import of the glucocorticoid receptor

Fri Apr 09 00:00:00 EDT 1999

Restoration of the glucocorticoid receptor function by the phosphodiester compound of vitamins C and E, EPC-K1 (L-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl hydrogen phosphate] potassium salt), via a redox-dependent mechanism.

Biochemical pharmacology

Okamoto K,Tanaka H,Makino Y,Makino I

PA1-512 was used in western blot to investigate the effect of redox state on glucocorticoid receptor function and the effect of antioxidants

Wed Jul 01 00:00:00 EDT 1998

RAP46 is a negative regulator of glucocorticoid receptor action and hormone-induced apoptosis.

The Journal of biological chemistry

Kullmann M,Schneikert J,Moll J,Heck S,Zeiner M,Gehring U,Cato AC

PA1-512 was used in EMSA assay to study the effect of RAP46 on glucocorticoid receptor action and hormone-induced apoptosis

Fri Jun 05 00:00:00 EDT 1998

Transrepression of c-jun gene expression by the glucocorticoid receptor requires both AP-1 sites in the c-jun promoter.

Molecular endocrinology (Baltimore, Md.)

Wei P,Inamdar N,Vedeckis WV

PA1-512 was used in western blot to study the role of the glucocorticoid during the modulation of the c-jun promoter activity

Tue Sep 01 00:00:00 EDT 1998

Thioredoxin: a redox-regulating cellular cofactor for glucocorticoid hormone action. Cross talk between endocrine control of stress response and cellular antioxidant defense system.

The Journal of clinical investigation

Makino Y,Okamoto K,Yoshikawa N,Aoshima M,Hirota K,Yodoi J,Umesono K,Makino I,Tanaka H

PA1-512 was used in western blot to investigate the role of thioredoxin (TRX) on cellular sensitivity to glucocorticoids

Sun Dec 01 00:00:00 EST 1996

Charge heterogeneity in wildtype and variant glucocorticoid receptors.

Molecular and cellular endocrinology

Rowan BG,Ip MM

PA1-512 was used in western blot to study the charge heterogeneity of glucocorticoid receptors from lymphosarcoma lines sensitive and resistant to glucocorticoid-mediated apoptosis

Sun Jan 01 00:00:00 EST 1995

Ligand-dependent down-regulation of stably transfected human glucocorticoid receptors is associated with the loss of functional glucocorticoid responsiveness.

Molecular endocrinology (Baltimore, Md.)

Bellingham DL,Sar M,Cidlowski JA

PA1-512 was used in western blot to investigate the regulation of stably transfected human glucocorticoid receptors by glucocorticoids

Tue Dec 01 00:00:00 EST 1992

Immunocytochemical localization of the glucocorticoid receptor in rat brain, pituitary, liver, and thymus with two new polyclonal antipeptide antibodies.

Endocrinology

McGimsey WC,Cidlowski JA,Stumpf WE,Sar M

PA1-512 was used in immunocytochemistry to investigate the distribution of glucocorticoid receptor in various rat tissues

Sun Dec 01 00:00:00 EST 1991

Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors.

Molecular endocrinology (Baltimore, Md.)

Cidlowski JA,Bellingham DL,Powell-Oliver FE,Lubahn DB,Sar M

PA1-512 was used in western blot to produce novel antibodies specifically recognizing human GR

Mon Oct 01 00:00:00 EDT 1990

Human glucocorticoid receptor cDNA contains sequences sufficient for receptor down-regulation.

The Journal of biological chemistry

Burnstein KL,Jewell CM,Cidlowski JA

PA1-512 was used in western blot to investigate the intrinsic mechanism for the down-regulation of glucocorticoid receptor expression

Sat May 05 00:00:00 EDT 1990

Bioinformatics

Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858

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Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1

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UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536

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Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding glucocorticoid receptor activity steroid binding protein binding zinc ion binding protein complex binding glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity steroid hormone binding RNA polymerase II regulatory region DNA binding DNA binding chromatin binding double-stranded DNA binding steroid hormone receptor activity transcription coactivator activity transcription factor binding lipid binding Hsp70 protein binding receptor tyrosine kinase binding heat shock protein binding hormone binding protein homodimerization activity sequence-specific DNA binding metal ion binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding

Process(es)

regulation of gluconeogenesis chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glucocorticoid metabolic process regulation of glucose metabolic process positive regulation of glutamate secretion chromatin modification adrenal gland development negative regulation of synaptic plasticity regulation of glucocorticoid biosynthetic process regulation of cell proliferation maternal behavior glucocorticoid receptor signaling pathway negative regulation of vascular permeability steroid hormone mediated signaling pathway glucocorticoid mediated signaling pathway positive regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter response to arsenic-containing substance chromatin-mediated maintenance of transcription mammary gland duct morphogenesis positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to steroid hormone stimulus cellular response to dexamethasone stimulus negative regulation of glucocorticoid mediated signaling pathway positive regulation of glucocorticoid receptor signaling pathway brain development aging circadian rhythm androgen metabolic process response to radiation response to activity response to inactivity muscle atrophy lung development response to insulin response to mercury ion response to corticosterone response to calcium ion response to electrical stimulus cellular response to magnesium ion response to dexamethasone transcription initiation from RNA polymerase II promoter apoptotic process chromosome segregation mitotic nuclear division signal transduction covalent chromatin modification cell division

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Glucocorticoid Receptor Polyclonal Antibody

Glucocorticoid Receptor Antibody (PA1-512) in ICC/IF

Product Details

PA1-512

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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