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Invitrogen
Glucocorticoid Receptor Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Product Details
PA5-21341
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 265 of Glucocorticoid Receptor beta (Uniprot ID#P04150)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11152512
Product Specific Information
Recommended positive controls: HeLa, A431, Mouse heart, Rat heart.
Predicted reactivity: Dog (88%), Cat (89%), Pig (83%), Sheep (89%), Bovine (90%), Guinea pig (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536
Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
glucocorticoid receptor activity
steroid binding
protein binding
zinc ion binding
protein complex binding
glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity
steroid hormone binding
RNA polymerase II regulatory region DNA binding
DNA binding
chromatin binding
double-stranded DNA binding
steroid hormone receptor activity
transcription coactivator activity
transcription factor binding
lipid binding
Hsp70 protein binding
receptor tyrosine kinase binding
heat shock protein binding
hormone binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
protein dimerization activity
Hsp90 protein binding
regulation of gluconeogenesis
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
glucocorticoid metabolic process
regulation of glucose metabolic process
positive regulation of glutamate secretion
chromatin modification
adrenal gland development
negative regulation of synaptic plasticity
regulation of glucocorticoid biosynthetic process
regulation of cell proliferation
maternal behavior
glucocorticoid receptor signaling pathway
negative regulation of vascular permeability
steroid hormone mediated signaling pathway
glucocorticoid mediated signaling pathway
positive regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
response to arsenic-containing substance
chromatin-mediated maintenance of transcription
mammary gland duct morphogenesis
positive regulation of dendritic spine development
positive regulation of cell growth involved in cardiac muscle cell development
cellular response to steroid hormone stimulus
cellular response to dexamethasone stimulus
negative regulation of glucocorticoid mediated signaling pathway
positive regulation of glucocorticoid receptor signaling pathway
brain development
aging
circadian rhythm
androgen metabolic process
response to radiation
response to activity
response to inactivity
muscle atrophy
lung development
response to insulin
response to mercury ion
response to corticosterone
response to calcium ion
response to electrical stimulus
cellular response to magnesium ion
response to dexamethasone
transcription initiation from RNA polymerase II promoter
apoptotic process
chromosome segregation
mitotic nuclear division
signal transduction
covalent chromatin modification
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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