Invitrogen

Glucocorticoid receptor (NR3C1) Monoclonal Antibody (BuGR2), APC, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (67) Glucocorticoid Receptor (Nr3c1) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

17-6189-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BuGR2

Immunogen

Partially purified rat GR
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811764

Product Specific Information

Description: This BuGR2 monoclonal antibody reacts with mouse glucocorticoid receptor NR3CR1. This clone has been also reported to cross-react with human NR3CR1. This BuGR2 antibody is recommended to be used with the Foxp3/Transcription factor staining buffer set (Product # 00-5523-00).

Applications Reported: This BuGR2 antibody has been reported for use in flow cytometric analysis. This clone has been also reported to cross-react with Human, Non-human primate, Rabbit, Rat, Sheep, Xenopus, Yeast NR3CR1.

Applications Tested: This BuGR2 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; OTTHUMP00000222854; OTTHUMP00000222858

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Gene Aliases: GR; Grl; Grl-1; Grl1; Nr3c1

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UniProt ID: (Mouse) P06537

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Entrez Gene ID: (Mouse) 14815

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity glucocorticoid receptor activity steroid binding protein binding transcription factor binding zinc ion binding lipid binding Hsp70 protein binding receptor tyrosine kinase binding heat shock protein binding protein complex binding glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity hormone binding protein homodimerization activity sequence-specific DNA binding metal ion binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding steroid hormone binding
Process(es)
regulation of gluconeogenesis chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glucocorticoid metabolic process regulation of glucose metabolic process positive regulation of glutamate secretion chromatin modification adrenal gland development negative regulation of synaptic plasticity regulation of glucocorticoid biosynthetic process regulation of cell proliferation maternal behavior glucocorticoid receptor signaling pathway negative regulation of vascular permeability steroid hormone mediated signaling pathway glucocorticoid mediated signaling pathway positive regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter response to arsenic-containing substance chromatin-mediated maintenance of transcription mammary gland duct morphogenesis positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to steroid hormone stimulus cellular response to dexamethasone stimulus negative regulation of glucocorticoid mediated signaling pathway positive regulation of glucocorticoid receptor signaling pathway
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