Proteintech

Glutamine synthetase Polyclonal Antibody

Glutamine synthetase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

Glutamine synthetase Antibody (11037-2-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 11037-2-AP) Glutamine Synthetase antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Glutamine synthetase Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Glutamine synthetase Antibody in Western Blot (WB)

Glutamine synthetase Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

11037-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Glutamine synthetase Fusion Protein Ag1510 (1-373 aa encoded by BC011700)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTTSASSHL NKGIKQVYMS LPQGEKVQAM YIWIDGTGEG LRCKTRTLDS EPKCVEELPE WNFDGSSTLQ SEGSNSDMYL VPAAMFRDPF RKDPNKLVLC EVFKYNRRPA ETNLRHTCKR IMDMVSNQHP WFGMEQEYTL MGTDGHPFGW PSNGFPGPQG PYYCGVGADR AYGRDIVEAH YRACLYAGVK IAGTNAEVMP AQWEFQIGPC EGISMGDHLW VARFILHRVC EDFGVIATFD PKPIPGNWNG AGCHTNFSTK AMREENGLKY IEEAIEKLSK RHQYHIRAYD PKGGLDNARR LTGFHETSNI NDFSAGVANR SASIRIPRTV GQEKKGYFED RRPSANCDPF SVTEALIRTC LLNETGDEPF QYKN (1-373 aa encoded by BC011700)

Target Information

Glutamine synthase is part of the glutamine synthetase family. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of GS in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of GS is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of hepatic manganese transporter ZIP8 disrupts serum transferrin glycosylation and the glutamate-glutamine cycle.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

Powers M,Minchella D,Gonzalez-Acevedo M,Escutia-Plaza D,Wu J,Heger C,Milne G,Aschner M,Liu Z

Sat Jul 01 00:00:00 EDT 2023

A role of GABA_A receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine.

Neuropharmacology

Tang XH,Diao YG,Ren ZY,Zang YY,Zhang GF,Wang XM,Duan GF,Shen JC,Hashimoto K,Zhou ZQ,Yang JJ

Wed Mar 01 00:00:00 EST 2023

A role of GABA_A receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine.

Neuropharmacology

Tang XH,Diao YG,Ren ZY,Zang YY,Zhang GF,Wang XM,Duan GF,Shen JC,Hashimoto K,Zhou ZQ,Yang JJ

Wed Mar 01 00:00:00 EST 2023

Spatially resolved isotope tracing reveals tissue metabolic activity.

Nature methods

Wang L,Xing X,Zeng X,Jackson SR,TeSlaa T,Al-Dalahmah O,Samarah LZ,Goodwin K,Yang L,McReynolds MR,Li X,Wolff JJ,Rabinowitz JD,Davidson SM

Tue Feb 01 00:00:00 EST 2022

Insm1 promotes differentiation of retinal progenitor cells toward photoreceptor cells in the developing retina through up-regulation of SHH.

Journal of molecular histology

Zhou P,Xu Y,Yang L,Meng P,Guo J,Liu Z,Jin B,Xie K,Zhu H,Du L,Jin X

Thu Dec 01 00:00:00 EST 2022

Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.

Frontiers in cell and developmental biology

Bai C,Wang H,Dong D,Li T,Yu Z,Guo J,Zhou W,Li D,Yan R,Wang L,Wang Z,Li Y,Ren L

Wed Apr 21 00:00:00 EDT 2021

PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.

Experimental eye research

Guo Y,Cang X,Zhu L,Zhu M,Li A,Wang Z,Zhang Y,Wang X,Song E

Wed Sep 01 00:00:00 EDT 2021

An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.

Science translational medicine

Li WJ,Zhu XJ,Yuan TJ,Wang ZY,Bian ZQ,Jing HS,Shi X,Chen CY,Fu GB,Huang WJ,Shi YP,Liu Q,Zeng M,Zhang HD,Wu HP,Yu WF,Zhai B,Yan HX

Wed Jul 08 00:00:00 EDT 2020

Enhanced Contextual Fear Memory and Elevated Astroglial Glutamate Synthase Activity in Hippocampal CA1 BChE shRNA Knockdown Mice.

Frontiers in psychiatry

Chen S,Lin Z,Tan KL,Chen R,Su W,Zhao H,Tan Q,Tan W

Thu Oct 22 00:00:00 EDT 2020

Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.

Journal of medicinal food

Li H,Liang M,Wang Z,Zhang Y,Wu Q,Yang L

Mon Jun 01 00:00:00 EDT 2020

α-ketobutyrate links alterations in cystine metabolism to glucose oxidation in mtDNA mutant cells.

Metabolic engineering

Lesner NP,Gokhale AS,Kota K,DeBerardinis RJ,Mishra P

Wed Jul 01 00:00:00 EDT 2020

Eurocristatine, a plant alkaloid from Eurotium cristatum, alleviates insulin resistance in db/db diabetic mice via activation of PI3K/AKT signaling pathway.

European journal of pharmacology

Zhang H,Hui J,Yang J,Deng J,Fan D

Sun Nov 15 00:00:00 EST 2020

Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.

Journal of food biochemistry

Wang Z,Cai L,Li H,Liang M,Zhang Y,Wu Q,Yang L

Fri May 01 00:00:00 EDT 2020

Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis.

Evidence-based complementary and alternative medicine : eCAM

Nie S,Zhao Y,Qiu X,Wang W,Yao Y,Yi M,Wang D

Wed Sep 30 00:00:00 EDT 2020

Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.

BMC cancer

Nakamura N,Hatano E,Iguchi K,Sato M,Kawaguchi H,Ohtsu I,Sakurai T,Aizawa N,Iijima H,Nishiguchi S,Tomono T,Okuda Y,Wada S,Seo S,Taura K,Uemoto S,Ikegawa M

Tue Jun 25 00:00:00 EDT 2019

Androgens regulate follicle stage-dependent pro- and anti-apoptosis in teleost ovaries through ZIP9 activation of different G proteins†.

Biology of reproduction

Converse A,Thomas P

Thu Aug 01 00:00:00 EDT 2019

Androgens regulate follicle stage-dependent pro- and anti-apoptosis in teleost ovaries through ZIP9 activation of different G proteins†.

Biology of reproduction

Converse A,Thomas P

Thu Aug 01 00:00:00 EDT 2019

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Long-Term Moderate Exercise Rescues Age-Related Decline in Hippocampal Neuronal Complexity and Memory.

Gerontology

Tsai SF,Ku NW,Wang TF,Yang YH,Shih YH,Wu SY,Lee CW,Yu M,Yang TT,Kuo YM

Wed Jan 30 00:00:00 EST 2019

Long-Term Moderate Exercise Rescues Age-Related Decline in Hippocampal Neuronal Complexity and Memory.

Gerontology

Tsai SF,Ku NW,Wang TF,Yang YH,Shih YH,Wu SY,Lee CW,Yu M,Yang TT,Kuo YM

Wed Jan 30 00:00:00 EST 2019

Ceftriaxone Improves Cognitive Function and Upregulates GLT-1-Related Glutamate-Glutamine Cycle in APP/PS1 Mice.

Journal of Alzheimer's disease : JAD

Fan S,Xian X,Li L,Yao X,Hu Y,Zhang M,Li W

Mon Nov 25 00:00:00 EST 2019

Ceftriaxone Improves Cognitive Function and Upregulates GLT-1-Related Glutamate-Glutamine Cycle in APP/PS1 Mice.

Journal of Alzheimer's disease : JAD

Fan S,Xian X,Li L,Yao X,Hu Y,Zhang M,Li W

Mon Nov 25 00:00:00 EST 2019

High-fat diet suppresses the astrocytic process arborization and downregulates the glial glutamate transporters in the hippocampus of mice.

Brain research

Tsai SF,Wu HT,Chen PC,Chen YW,Yu M,Wang TF,Wu SY,Tzeng SF,Kuo YM

Sat Dec 01 00:00:00 EST 2018

High-fat diet suppresses the astrocytic process arborization and downregulates the glial glutamate transporters in the hippocampus of mice.

Brain research

Tsai SF,Wu HT,Chen PC,Chen YW,Yu M,Wang TF,Wu SY,Tzeng SF,Kuo YM

Sat Dec 01 00:00:00 EST 2018

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

Journal of translational medicine

Han Q,Ma Y,Wang H,Dai Y,Chen C,Liu Y,Jing L,Sun X

Fri Jul 20 00:00:00 EDT 2018

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

Journal of translational medicine

Han Q,Ma Y,Wang H,Dai Y,Chen C,Liu Y,Jing L,Sun X

Fri Jul 20 00:00:00 EDT 2018

Cerebral level of vGlut1 is increased and level of glycine is decreased in TgSwDI mice.

Journal of Alzheimer's disease : JAD

Timmer NM,Metaxas A,van der Stelt I,Kluijtmans LA,van Berckel BN,Verbeek MM

Wed Sep 03 00:00:00 EDT 2014

Cerebral level of vGlut1 is increased and level of glycine is decreased in TgSwDI mice.

Journal of Alzheimer's disease : JAD

Timmer NM,Metaxas A,van der Stelt I,Kluijtmans LA,van Berckel BN,Verbeek MM

Wed Sep 03 00:00:00 EDT 2014

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Proceedings of the National Academy of Sciences of the United States of America

Liu W,Le A,Hancock C,Lane AN,Dang CV,Fan TW,Phang JM

Tue Jun 05 00:00:00 EDT 2012

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Proceedings of the National Academy of Sciences of the United States of America

Liu W,Le A,Hancock C,Lane AN,Dang CV,Fan TW,Phang JM

Tue Jun 05 00:00:00 EDT 2012

Optimisation of the quantification of glutamine synthetase and myelin basic protein in cerebrospinal fluid by a combined acidification and neutralisation protocol.

Journal of immunological methods

Herbert MK,Kuiperij HB,Verbeek MM

Tue Jul 31 00:00:00 EDT 2012

Optimisation of the quantification of glutamine synthetase and myelin basic protein in cerebrospinal fluid by a combined acidification and neutralisation protocol.

Journal of immunological methods

Herbert MK,Kuiperij HB,Verbeek MM

Tue Jul 31 00:00:00 EDT 2012

Histologic subtypes of hepatoblastoma are characterized by differential canonical Wnt and Notch pathway activation in DLK+ precursors.

Human pathology

López-Terrada D,Gunaratne PH,Adesina AM,Pulliam J,Hoang DM,Nguyen Y,Mistretta TA,Margolin J,Finegold MJ

Mon Jun 01 00:00:00 EDT 2009

Histologic subtypes of hepatoblastoma are characterized by differential canonical Wnt and Notch pathway activation in DLK+ precursors.

Human pathology

López-Terrada D,Gunaratne PH,Adesina AM,Pulliam J,Hoang DM,Nguyen Y,Mistretta TA,Margolin J,Finegold MJ

Mon Jun 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: cell proliferation-inducing protein 59; GLNA; Glutamate ammonia ligase; glutamate decarboxylase; Glutamate--ammonia ligase; glutamate-ammonia ligase (glutamine synthase); glutamate-ammonia ligase (glutamine synthetase); glutamine synthase; Glutamine synthetase; Glutamine synthetase (glutamate-ammonia ligase); glutamine synthetase 1; glutamine synthetase I; GS; Palmitoyltransferase GLUL; Proliferation inducing protein 43; proliferation-inducing protein 43

View more View less

Gene Aliases: GLNS; GLUL; GS; PIG43; PIG59

View more View less

UniProt ID: (Human) P15104, (Mouse) P15105, (Rat) P09606

View more View less

Entrez Gene ID: (Human) 2752, (Mouse) 14645, (Rat) 24957

View more View less

Function(s)

magnesium ion binding glutamate decarboxylase activity glutamate-ammonia ligase activity protein binding ATP binding glutamate binding manganese ion binding identical protein binding dynein light chain binding nucleotide binding catalytic activity lyase activity ligase activity ligase

Process(es)

neurotransmitter uptake glutamate catabolic process glutamine biosynthetic process cell proliferation cellular amino acid biosynthetic process cellular response to starvation response to glucose ammonia assimilation cycle positive regulation of insulin secretion positive regulation of epithelial cell proliferation protein homooligomerization positive regulation of synaptic transmission, glutamatergic glutamate metabolic process nitrogen compound metabolic process neurotransmitter metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Glutamine synthetase Polyclonal Antibody

Glutamine synthetase Antibody (11037-2-AP) in IHC (P)

Product Details

11037-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!