Invitrogen

Biotin Polyclonal Antibody

Biotin Antibody in Immunocytochemistry (ICC/IF)

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Biotin Antibody (31852) in ICC/IF

Nature communications 2021 - Immobile IP 3 Rs associate with actin and KRAP. a Numbers of Ca 2+ puffs and Ca 2+ -release sites detected in EGFP-IP 3 R1 HeLa cells in the 20 s after photolysis of ci-IP 3 with or without extracellular Ca 2+ (2.5 mM BAPTA added immediately before UV flash). Mean +- s.d., 8 cells. b Mean puff amplitudes for individual cells (mean +- s.e.m., n = 8 cells). The results establish that Ca 2+ leaking across the ... View More

Biotin Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

31852

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:5,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

In Situ Hybridization (ISH) (ISH)

Assay-dependent

Product Specifications

Species Reactivity

Chemical

Published species

Bacteria, Chemical, Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Biotin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 20mg/mL sucrose

Contains

0.08% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_228243

Product Specific Information

The activity measured by a solid phase binding assay is 99%.

Reconstitute using 1 mL of distilled water; product may be stored for several weeks as an undiluted liquid. After dilution, do not use for more than one day. After one year, the reconstituted liquid product should be stored frozen in small undiluted aliquots, avoiding freeze/thaw cycles.

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heat denaturation enables multicolor X10-STED microscopy.

Scientific reports

Saal KA,Shaib AH,Mougios N,Crzan D,Opazo F,Rizzoli SO

31852 was used in Immunohistochemistry-immunofluorescence to present a expansion microscopy protocol that ensures the expansion of the samples up to 10-fold, in a single expansion step, through high-temperature homogenization (X10ht).

Sat Apr 01 00:00:00 EDT 2023

ALS-linked loss of Cyclin-F function affects HSP90.

Life science alliance

Siebert A,Gattringer V,Weishaupt JH,Behrends C

31852 was used in Immunocytochemistry, Immunohistochemistry, In Situ Hybridization, Western Blot to suggest a possible Cyclin-F loss-of-function-mediated chaperone dysregulation that might be relevant for ALS.

Fri Sep 16 00:00:00 EDT 2022

Cathepsin B Relocalization in Late Membrane Disrupted Neurons Following Diffuse Brain Injury in Rats.

ASN neuro

Hernandez ML,Marone M,Gorse KM,Lafrenaye AD

31852 was used in Immunocytochemistry-immunofluorescence to investigate the localization and activity of lysosomal cathepsin B in neurons with disrupted plasmalemmal membranes weeks following diffuse brain injury.

Thu May 05 00:00:00 EDT 2022

Quantitative transportomics identifies Kif5a as a major regulator of neurodegeneration.

eLife

Shah SH,Schiapparelli LM,Ma Y,Yokota S,Atkins M,Xia X,Cameron EG,Huang T,Saturday S,Sun CB,Knasel C,Blackshaw S,Yates JR,Cline HT,Goldberg JL

31852 was used in Western Blot to analyse early changes in the protein 'transportome' from retinal ganglion cells(RGC) somas to their axons after optic nerve injury and identified transport failure of an anterograde motor protein Kif5a early in RGC degeneration.

Tue Mar 08 00:00:00 EST 2022

KRAP tethers IP₃ receptors to actin and licenses them to evoke cytosolic Ca²⁺ signals.

Nature communications

Thillaiappan NB,Smith HA,Atakpa-Adaji P,Taylor CW

Published figure using Biotin polyclonal antibody (Product # 31852) in Immunocytochemistry

Fri Jul 23 00:00:00 EDT 2021

KRAP tethers IP₃ receptors to actin and licenses them to evoke cytosolic Ca²⁺ signals.

Nature communications

Thillaiappan NB,Smith HA,Atakpa-Adaji P,Taylor CW

Published figure using Biotin polyclonal antibody (Product # 31852) in Immunocytochemistry

Fri Jul 23 00:00:00 EDT 2021

Buprenorphine alters microglia and astrocytes acutely following diffuse traumatic brain injury.

Scientific reports

Ryu J,Stone P,Lee S,Payne B,Gorse K,Lafrenaye A

31852 was used in Immunohistochemistry to suggest effects of Bup treatment on weight following CFPI and potential regional specificity of Bup-associated microglial and astrocyte alterations, but very little change in other acute pathology at 1-day post-injury.

Wed Apr 21 00:00:00 EDT 2021

Synthesis of d-desthiobiotin-AI-2 as a novel chemical probe for autoinducer-2 quorum sensing receptors.

Bioorganic chemistry

Miranda V,Torcato IM,Xavier KB,Ventura MR

31852 was used in Western Blot to highlight the potential of conjugating chemical signals to biotinylated derivatives to identify and tag signal receptors involved in quorum sensing or other chemical signalling processes.

Fri Nov 01 00:00:00 EDT 2019

Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.

Scientific reports

Richter KN,Patzelt C,Phan NTN,Rizzoli SO

31852 was used in Immunocytochemistry to study whether the organelle proteins colocalize when they are on the plasma membrane, or whether they disperse synaptic vesicles, organelles that are known to exo- and endocytose frequently along with immunostainings for other synaptic molecules.

Tue Jun 25 00:00:00 EDT 2019

Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells.

Nucleic acids research

Vella P,Barozzi I,Cuomo A,Bonaldi T,Pasini D

Published figure using Biotin polyclonal antibody (Product # 31852) in Western Blot

Sun Apr 01 00:00:00 EDT 2012

Alkylation of the tumor suppressor PTEN activates Akt and β-catenin signaling: a mechanism linking inflammation and oxidative stress with cancer.

PloS one

Covey TM,Edes K,Coombs GS,Virshup DM,Fitzpatrick FA

31852 was used in western blot to investigate the effects of PTEN alkylation

Thu Oct 21 00:00:00 EDT 2010

Alkylation of the tumor suppressor PTEN activates Akt and β-catenin signaling: a mechanism linking inflammation and oxidative stress with cancer.

PloS one

Covey TM,Edes K,Coombs GS,Virshup DM,Fitzpatrick FA

31852 was used in western blot to investigate the effects of PTEN alkylation

Thu Oct 21 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Vitamin B4

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Biotin Polyclonal Antibody

Biotin Antibody (31852) in ICC/IF

Product Details

31852

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

In Situ Hybridization (ISH) (ISH)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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