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Invitrogen
Goat anti-Human IgA (Heavy chain) Secondary Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Human IgA (Heavy chain) Secondary Antibody (31140) in WB
Western blot was performed using Goat anti-Human IgA (Heavy chain) Secondary Antibody (Product # 31140) and a 55 kDa band corresponding to Human IgA Heavy Chain was observed in IgA but not in IgG1, IgG2, IgG3, IgG4, IgM, IgE, Mouse IgG, IgM and Rabbit IgG. Purified protein (100 ng) of Human IgG1 (Lane 1), IgG2 (Lane 2), IgG3 (Lane 3), IgG4 (Lane 4), IgA (Lane 5), IgM (Lane 6), IgE (Lane 7), Mouse IgG (Lane ... View More
Product Details
31140
Applications
Tested Dilution
Western Blot (WB)
1 µg/mL
Immunohistochemistry (IHC)
Assay-dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
Assay-dependent
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228251
Target
IgA (Heavy chain)
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31140 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31140 reacts with the heavy chain portion of human IgA, but not with the heavy chains of human IgG or IgM. The antibody does not react against the light chains common to most human immunoglobulins, or against non-immunoglobulin serum proteins. However, this antibody may cross-react with IgA from other species.
Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.
Country of Origin: USA
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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