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Invitrogen
Goat anti-Human IgA Secondary Antibody, HRP
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Human IgA Secondary Antibody (PA1-74395) in WB
Western blot was performed using Goat anti-Human IgA Secondary Antibody, HRP (Product # PA1-74395) and a 55 kDa band corresponding to Human IgA Heavy Chain was observed in IgA but not in IgG1, IgG2, IgG3, IgG4, IgM, IgE, Mouse IgG, IgM and Rabbit IgG. Purified protein (100 ng) of Human IgG1 (Lane 1), IgG2 (Lane 2), IgG3 (Lane 3), IgG4 (Lane 4), IgA (Lane 5), IgM (Lane 6), IgE (Lane 7), Mouse IgG (Lane 8), Mouse IgM (Lane 9) and Rabbit IgG (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolve... View More
Product Details
PA1-74395
Applications
Tested Dilution
Western Blot (WB)
1:2000
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
ELISA (ELISA)
1/4000 - 1/8000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Human IgA paraproteins.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10985660
Target
IgA
Antibody Form
Whole Antibody
Product Specific Information
Goat anti human IgA polyclonal antibody recognizes the heavy chain of human IgA. This anti human IgA antibody has been cross adsorbed against human IgM and IgG but might cross-react with IgA from other species.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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