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Invitrogen
Goat anti-Human IgG F(ab')2 Secondary Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Human IgG F(ab')2 Secondary Antibody (31122) in ICC/IF
Immunofluorescence analysis of Goat anti-Human IgG F(ab)2 Secondary Antibody was performed using log phase IM9 cells (IgG producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Goat anti-Human IgG F(ab)2 Secondary Antibody (Product # 31122) a... View More
Product Details
31122
Applications
Tested Dilution
Western Blot (WB)
1 ug/ml
Immunohistochemistry (IHC)
Assay-dependent
Immunocytochemistry (ICC/IF)
1:100
Flow Cytometry (Flow)
Assay-dependent
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228271
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31122 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31122 reacts with the light chains of human IgG and with the light chains common to other human immunoglobulins, but not with the Fc portion of human IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.
Country of origin: USA
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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