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Invitrogen
Goat anti-Human IgG Fab Superclonal™ Secondary Antibody, Alexa Fluor™ 488
Advanced Verification
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Human IgG Fab Secondary Antibody (A66729) in ICC/IF
Immunofluorescence analysis of Goat-anti-Human IgG Fab, Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # A66729) was performed on fixed and permeabilized T-47D cells blocked with 2% BSA at room temperature and stained with MUC-1/Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157, used at 1:500 dilution) in 0.1% BSA overnight at 4-degree Celsius. This was detecte... View More
Product Details
A66729
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
2 µg/mL
Immunocytochemistry (ICC/IF)
1:500-1:1,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Purified Human IgG
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
5mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Target
IgG Fab
Antibody Form
Whole Antibody
Product Specific Information
This Goat-anti-Human IgG Fab, Superclonal™ Recombinant Secondary Antibody detects human IgG Fab with specificity to the light chains, kappa and lambda, as well as the heavy chain CH1. This antibody does not detect the Fc and does not cross-react with immunoglobulins from other species.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency.
Superclonal™ Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.
The sensitivity and specificity of each lot is confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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