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Invitrogen
F(ab')2-Goat anti-Human IgG Fc gamma Secondary Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Human IgG Fc gamma Secondary Antibody (31163) in ICC/IF
Immunofluorescence analysis of F(ab)2-Goat anti-Human IgG Fc gamma Secondary Antibody was performedusing log phase IM9 cells (IgG producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes,permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cellswere labeled with F(ab)2-Goat anti-Human IgG Fc gamma Secondary Antibody (Pro... View More
Product Details
31163
Applications
Tested Dilution
Western Blot (WB)
1:2000
Immunohistochemistry (IHC)
Assay-dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
Assay-dependent
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228214
Target
IgG Fc
Antibody Form
F(ab')2 Fragment
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31163 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31163 reacts with the heavy chains of human IgG, but not with the light chains to most human immunoglobulins or with non-immunoglobulin serum proteins. This antibody does not react against human IgM or IgA. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. After opening, dilute only enough product for a single day's use. Store remainder at 2-8 °C under sterile conditions.
Country of Origin: USA
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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