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Invitrogen
Goat anti-Human IgG (Light chain) Superclonal™ Secondary Antibody
Advanced Verification
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 16
Human IgG (Light chain) Secondary Antibody (A56853) in ICC/IF
Immunofluorescence analysis of Goat anti-Human IgG (Light Chain Kappa+Lambda), Superclonal™ Recombinant Secondary Antibody (Product # A56853) was performed on fixed and permeabilized SK-BR-3 cells stained with HER2/Trastuzumab Recombinant Human Monoclonal Antibody (Product# MA5-42305, 1:100) followed by Goat anti-Human IgG (Light Chain Kappa+Lambda), Superclonal™ Recombinant Secondary Antibody (Product # A5... View More
Product Details
A56853
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:500-1:1,000
ELISA (ELISA)
1:1,000-1:4,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Purified Human IgG
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2930919
Target
IgG (Light chain)
Antibody Form
Whole Antibody
Product Specific Information
This Goat anti-Human IgG (Light Chain Kappa+Lambda), Superclonal™ Recombinant Secondary Antibody specifically detects both Kappa and the Lambda light chains of human IgG, with no cross-reactivity to heavy chains and other species immunoglobulins.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency.
Superclonal™ Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.
The sensitivity and specificity of each lot is confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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