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Invitrogen
Goat anti-Human IgM (Heavy chain) Recombinant Secondary Antibody
Advanced Verification
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Human IgM (Heavy chain) Secondary Antibody (A54083) in WB
Western blot analysis of Goat anti-Human IgM (Heavy chain), Recombinant Monoclonal Antibody (Product # A54083) was performed under reducing conditions by loading purified human IgM at concentrations of 0.125µg (Lane 1), 0.25µg (Lane 2), 0.5µg (Lane 3) and 1µg (Lane 4) and were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitroce... View More
Product Details
A54083
Applications
Tested Dilution
Western Blot (WB)
1:5000
ELISA (ELISA)
1:1000 to 1:2000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
22H20L13
Immunogen
Purified human IgM
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2910648
Target
IgM (Heavy chain)
Antibody Form
Whole Antibody
Product Specific Information
This Goat anti-Human IgM (Heavy chain), Recombinant Monoclonal Antibody reacts with human IgM heavy chain with no cross reactivity to human IgG or IgA or various species immunoglobulins, including monkey IgM.
The sensitivity and specificity of each lot is confirmed using ELISA.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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