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Invitrogen
Goat anti-Human IgM Secondary Antibody, Biotin
Product Details
31778
Applications
Tested Dilution
Western Blot (WB)
1:20,000-1:400,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
ELISA (ELISA)
1:100-1:500
In Situ Hybridization (ISH) (ISH)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.8, with 1mg/mL BSA
Contains
0.08% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient
RRID
AB_228278
Target
IgM
Antibody Form
Whole Antibody
Product Specific Information
Product # 31778 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.
Product # 31778 reacts with the heavy chains of human IgM, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rabbit IgM, swine IgM (25%) rat IgM (12%) guinea pig IgG (6%) mouse IgM (3%) dog IgM (1%), and it has minimal cross-reactivity with immunoglobulins from other species.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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