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Invitrogen
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
FIGURE: 1 / 12
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (31432) in WB
Chemiluminescent western blot was performed using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # 31432). Whole cell extracts of U-87 MG (Lane 1, 2, 3, 4), HeLa (Lane 5), Hep G2 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting... View More
Product Details
31432
Applications
Tested Dilution
Western Blot (WB)
1:10,000-1:100,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_228302
Target
IgG
Cross Adsorption
Against human, bovine and horse serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31432 has been successfully used in Western blot, and ICC applications.
Antibody Specificity: This antibody reacts with whole molecule of mouse IgG and with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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