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Invitrogen
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
FIGURE: 1 / 2
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (31802) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), K-562 (Lane 2). The blots were probed with Anti SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin (Product # 31802) at dilutions 1:50,000 (Fig. 1), 1:100,000 (Fig. 2) and 200,000 (Fig. 3). A 22 kDa ... View More
Product Details
31802
Applications
Tested Dilution
Western Blot (WB)
1:50,000-1:200,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
Flow Cytometry (Flow)
1:200-1:1,000
ELISA (ELISA)
1:20,000-1:400,000
Immunoprecipitation (IP)
1:500-1:5,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Lyophilized
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228301
Target
IgG
Cross Adsorption
Against human, bovine and horse serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31802 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31802 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins. However, these antibodies may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 1.5 mL of distilled water (1.2 mg/mL after restoration).
Country of Origin: USA
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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