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Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-31553) in ICC/IF
Microtubules of NIH 3T3 cells labeled with mouse anti-a-tubulin monoclonal IgG1 antibody (Product # A11126) and visualized with blue-fluorescent Alexa Fluor® 405 goat anti-mouse IgG antibody (Product # A-31553). Nuclei were stained with red-fluorescent propidium iodide (Product # P1304MP, P3566, P21493).
Product Details
A-31553
Applications
Tested Dilution
Immunohistochemistry (IHC)
1-10 µg/mL
Immunocytochemistry (ICC/IF)
1-10 µg/mL
Flow Cytometry (Flow)
1-10 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
401/422 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient
RRID
AB_221604
Target
IgG
Cross Adsorption
Against human IgG and serum prior to conjugation
Antibody Form
Whole Antibody
Product Specific Information
Product will be shipped at Room Temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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