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Invitrogen
F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, NovaFluor™ Red 685, eBioscience™
FIGURE: 1 / 2
Mouse IgG (H+L) Secondary Antibody (S001T03R02-A) in Flow
Staining of normal human peripheral blood cells with staining buffer (blue histogram) or Anti-Human CD4 Purified (a mouse IgG1 isotype, SK3, Product #603-041) (purple histogram) followed by 0.05 µg of F (ab)2 Anti-Mouse IgG NovaFluor Red 685 (Product # S001T03R02). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-l... View More
Product Details
S001T03R02-A
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
637/685 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3099272 (AB_2921197)
Target
IgG
Antibody Form
F(ab')2 Fragment
Product Specific Information
Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.IThis polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.
Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained first with a mouse anti-human antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This polyclonal antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded sections that have been stained first with a mouse anti-human antibody. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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