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Invitrogen
Goat anti-Mouse IgG, IgM (H+L) Secondary Antibody, AP
FIGURE: 1 / 1
Mouse IgG, IgM (H+L) Secondary Antibody (T2192) in WB
Protein expression in the muscle progenitor cells from the chicken embryo after 5 days of primary culture, determined according to the Western blot method. The figure shows the results for the control group and groups treated with l-glutamine (L-Glu), graphene oxide nanofilm (nGO) and nGO with addition of the L-Glu (nGOxL-Glu). The results represent a relative protein expression of the respective target pro... View More
Product Details
T2192
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunohistochemistry (IHC)
1:1,000
ELISA (ELISA)
1:1,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Mouse IgG/IgM
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
TBS with 40% glycerol, 4-5% BSA
Contains
0.05% sodium azide
Storage conditions
2-8°C
Shipping conditions
Ambient
RRID
AB_11180852
Target
IgG/IgM
Antibody Form
Whole Antibody
Product Specific Information
This Alkaline Phosphatase Conjugated secondary antibody can be used with the ELISA-Light Immunoassay, Western-Light, and Western-Star Western Blotting Detection Systems. This labeled secondary antibody ensures minimal nonspecific cross-reactivity. Thermo Scientific has optimized its alkaline phosphatase secondary antibody conjugates for Western blotting or immunoassay procedures incorporating chemiluminescent 1,2-dioxetane enzyme substrates. Optimized for use in blotting applications with dioxetane-based substrate solutions (CSPD or CDP-Star substrates), providing consistent experiment-to-experiment results. High specific activity of conjugates provides high sensitivity of detection with dioxetane-based substrate solutions (CSPD or CDP-Star substrates). Low non-specific binding provides highest possible signal-to-noise in western blotting and immunoassay applications. Easy to Use The recommended initial working dilution for the labeled antibodies is 1:5,000 for AP conjugates. The conjugate is tested and optimized for low background with chemiluminescent detection procedures.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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