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Invitrogen
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 514
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-31558) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 514 (Product # A-31558) was performed using MCF 10A (positive model) and T-47D (negative model) cells stained with Vimentin Polyclonal Antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA f... View More
Product Details
A-31558
Applications
Tested Dilution
Western Blot (WB)
1:10,000
Immunohistochemistry (IHC)
1-10 µg/mL
Immunocytochemistry (ICC/IF)
1-10 µg/mL
Flow Cytometry (Flow)
1-10 µg/mL
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
518/543 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient
RRID
AB_2536173
Target
IgG
Cross Adsorption
Against human IgG, human serum, mouse IgG and bovine serum
Antibody Form
Whole Antibody
Product Specific Information
The yellow-green fluorescent Alexa Fluor 514 dye is far superior to fluorescein in both brightness and photostability, and it can be detected with standard fluorescein, Oregon Green dye or Alexa Fluor 488 dye filter sets. By using instruments that collect spectral distribution information, the signal from the Alexa Fluor 514 dye can be distinguished from both the Alexa Fluor 488 and Alexa Fluor 500 dyes.
Product will be shipped at Room Temperature.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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