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Invitrogen
F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A10547) in WB
Chemiluminescent western blot was performed using F(ab)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # A10547). Membrane enriched extracts of THP1 (Lane 1, 2, 3, 4), MCF7 (Lane 5, 6, 7, 8) and HeLa (Lane 9) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB23001) byi... View More
Product Details
A10547
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:10,000
Immunohistochemistry (IHC)
1:500-1:2,000
ELISA (ELISA)
1:500-1:2,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_10561554
Target
IgG
Cross Adsorption
Against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins and purified human paraproteins
Antibody Form
F(ab')2 Fragment
Product Specific Information
Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.
Store product at -20°C until opened. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.
Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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