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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight™ 800 4X PEG
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 16
Rabbit IgG (H+L) Secondary Antibody (SA5-35571) in WB
Western blot analysis of Heat Shock Protein 90 (Hsp90) was performed by loading 2-fold serial dilutions of HeLa whole cell lysate (starting at 10 µg) and 10 µL of the PageRuler Prestained NIR Protein Ladder (Product # 26635) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to Low Fluorescence PVDF Membranes (Product # 22860) and blocked with SEA BLOCK Blocking Buffer (Product # 3... View More
Product Details
SA5-35571
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:30,000
Immunohistochemistry (IHC)
1:100-1:2,000
Immunocytochemistry (ICC/IF)
1:2,000
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified Rabbit IgG, whole molecule
Conjugate
Excitation/Emission Max
789/794 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
proprietary buffer
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2556775
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product SA5-35571 reacts with the heavy chains of Rabbit IgG and with the light chains common to most Rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol without and store at -20°C. Note: not compatible with sodium azide. Mole Dye/Mole Protein Ratio is lot-dependent.
DyLight 800-4xPEG has an excitation (nm) of 783 nm (Amax) and an emission (nm) of 797 nm (Emax) in PBS. The emission spectra for DyLight™ 800-4xPEG conjugate match the principal output wavelengths of most common fluorescence instrumentation.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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