Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 488
FIGURE: 1 / 4
Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10018) in ICC/IF
Immunofluorescent analysis of LAMP2 (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a LAMP2 monoclonal antibody (Product # MA1-165) at a dilution of 10 µg/mL in staining buffer for 1 hour at room temperatu... View More
Product Details
SA5-10018
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:20,000
Immunohistochemistry (IHC)
1:50-1:2,000
Immunocytochemistry (ICC/IF)
1:50-1:2,000
Flow Cytometry (Flow)
1:50-1:200
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Rat IgG-heavy and light chain
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 6.8 to 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2556598
Target
IgG
Cross Adsorption
Against bovine, chicken, human, mouse, rabbit and sheep
Antibody Form
Whole Antibody
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, human, mouse, rabbit and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support