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Invitrogen
Goat anti-Rat IgG (H+L) Secondary Antibody, HRP, eBioscience™
FIGURE: 1 / 2
Rat IgG (H+L) Secondary Antibody (18-4818-82) in WB
Mouse IL-27 Recombinant Protein (Product # 14-8271-63) (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane 3) were analyzed by SDS-PAGE followed by immunoblotting with 5 µg/mL of Anti-Mouse EBI3 (IL-27 subunit) Purified. Bands were visualized with Anti-Rat IgG HRP (Product # 18-4818-82).
Product Details
18-4818-82
Applications
Tested Dilution
Western Blot (WB)
1:10,000-1:100,000
Immunohistochemistry (IHC)
1:500-1:5,000
ELISA (ELISA)
1:5,000-1:100,000
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_469525
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Description: This is affinity purified HRP-conjugated Goat Anti-Rat IgG (H+L). This antibody has minimal cross-reactivity to Mouse, Human, Bovine, Horse, and Rabbit Serum Proteins. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rat IgG and with thelight chains common to most rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Applications Reported: HRP anti-rat IgG has been reported for use in immunoblotting (WB), immunohistochemical staining, and ELISA.
Applications Tested: This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, human,bovine, horse, and rabbit serum proteins, but the antibody may cross-react with immunoglobµLins from other species.Suggested dilution ranges are:1:500 - 1:5,000 for immunohistochemistry on tissue sections 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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