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Invitrogen
Golgin-97 Monoclonal Antibody (CDF4), eBioscience™
FIGURE: 1 / 6
Golgin-97 Antibody (14-9767-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized HeLa cells stained with 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Human Golgin-97 Purified (right) followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
Product Details
14-9767-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
CDF4
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573010
Product Specific Information
Description: The monoclonal antibody CDF4 recognizes human Golgin-97. Golgin-97, a GRIP domain protein member that was first identified as a Golgi complex autoantigen associated with Sjogren's syndrome. Golgin-97 is a peripheral membrane protein found on the trans face of the Golgi apparatus. The protein G family member, Arl1, mediates the binding of Golgin-97 to the Golgi membrane. Like all GRIP proteins, Golgin-97 functions in maintaining the integrity of the trans Golgi network. Golgin-97 has also been shown to function as a tethering molecule in the retrograde transport of proteins from the endosomes to the trans Golgi network. During poxvirus infection, Golgin-97 incorporates into virions and plays a role in viral replication and morphogenesis.
Applications Reported: This CDF4 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This CDF4 antibody has been tested by immunocytochemistry of fixed and permeabilized HeLa cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The Golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gap junction protein, alpha 4, 37kD; golgi autoantigen, golgin subfamily a, 1; Golgin subfamily A member 1; Golgin-97
Gene Aliases: GOLGA1; golgin-97
UniProt ID: (Human) Q92805
Entrez Gene ID: (Human) 2800
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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