Invitrogen

Granzyme B Monoclonal Antibody (GZB01)

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17 References

View all (87) Granzyme B antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Granzyme B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

Granzyme B Antibody (MA5-11587) in IHC (P)

Formalin-fixed, paraffin-embedded human lymphoma stained with Granzyme B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Granzyme B Antibody in Western Blot (WB)

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Product Details

MA5-11587

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GZB01

Immunogen

Recombinant protein encoding the N-terminus of the mature granzyme B

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10979667

Product Specific Information

MA5-11587 targets Granzyme B in IHC (P) applications and shows reactivity with Human samples.

The MA5-11587 immunogen is recombinant protein encoding the N-terminus of the mature granzyme B.

Target Information

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evidence of a pathogenic role for CD8(+) T cells in anti-GABAB receptor limbic encephalitis.

Neurology(R) neuroimmunology & neuroinflammation

Golombeck KS,Bönte K,Mönig C,van Loo KM,Hartwig M,Schwindt W,Widman G,Lindenau M,Becker AJ,Glatzel M,Elger CE,Wiendl H,Meuth SG,Lohmann H,Gross CC,Melzer N

MA5-11587 was used in immunohistochemistry to characterize anti-GABAB receptor limbic encephalitis and evidence of a pathogenic role for CD8(+) T cells

Wed Jun 01 00:00:00 EDT 2016

Autoimmune encephalitis in humans: how closely does it reflect multiple sclerosis ?

Acta neuropathologica communications

Höftberger R,Leisser M,Bauer J,Lassmann H

MA5-11587 was used in immunohistochemistry - paraffin section to compare multiple sclerosis to autoimmune encephalitis in humans

Fri Dec 04 00:00:00 EST 2015

CD30⁺ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Oncology letters

Yang YJ,Li YX,Liu YB,Yang M,Liu K

MA5-11587 was used in immunohistochemistry to report on a case of CD30-positive extranodal natural killer/T-cell lymphoma

Thu May 01 00:00:00 EDT 2014

Disease-specific molecular events in cortical multiple sclerosis lesions.

Brain : a journal of neurology

Fischer MT,Wimmer I,Höftberger R,Gerlach S,Haider L,Zrzavy T,Hametner S,Mahad D,Binder CJ,Krumbholz M,Bauer J,Bradl M,Lassmann H

MA5-11587 was used in immunohistochemistry - paraffin section to investigate the mechanisms that lead to demyelination and neurodegeneration

Sat Jun 01 00:00:00 EDT 2013

Clinicopathology, immunophenotype, T cell receptor gene rearrangement, Epstein-Barr virus status and p53 gene mutation of cutaneous extranodal NK/T-cell lymphoma, nasal-type.

Chinese medical journal

Wang TT,Xu C,Liu SL,Kan B,Ran YP,Liu WP,Li GD,Wang L

MA5-11587 was used in immunohistochemistry to study the potential association of a range of factors with nasal type cutaneous extranodal natural killer/T-cell lymphoma

Mon Apr 01 00:00:00 EDT 2013

Characterization of the inflammatory response to solid cancer metastases in the human brain.

Clinical & experimental metastasis

Berghoff AS,Lassmann H,Preusser M,Höftberger R

MA5-11587 was used in immunohistochemistry to study the inflammatory response to solid cancer metastases in the human brain

Tue Jan 01 00:00:00 EST 2013

Immunohistochemical expression of granzyme B and perforin in discoid lupus erythematosus.

Ultrastructural pathology

Abdou AG,Shoeib M,Bakry OA,El-Bality H

MA5-11587 was used in immunohistochemistry to study the clinicopathological significance of the expression of perforin and granzyme B in patients with discoid lupus erythematosus

Sun Dec 01 00:00:00 EST 2013

Invasion-associated genes identified by gene expression profiling in extranodal natural killer/T-cell lymphoma, nasal type.

Leukemia & lymphoma

Yu JB,Zhang YC,Yang QP,Wang XL,Tang Y,Zhao S,Mo XM,Liu WP

MA5-11587 was used in immunohistochemistry to identify invasion-associated genes in extranodal natural killer/T-cell lymphoma

Tue Jan 01 00:00:00 EST 2013

Leukocyte composition of human breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM

MA5-11587 was used in immunohistochemistry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery

Tue Feb 21 00:00:00 EST 2012

Tumorous proliferations of plasmacytoid dendritic cells and Langerhans cells associated with acute myeloid leukaemia.

Histopathology

Song HL,Huang WY,Chen YP,Chang KC

MA5-11587 was used in immunohistochemistry to study the association of tumorous proliferation of plasmacytoid dendritic cells and Langerhans cells with acute myeloid leukaemia

Thu Nov 01 00:00:00 EDT 2012

Hepatosplenic T-cell lymphoma: clinicopathologic, immunophenotypic, and molecular characterization of 17 Chinese cases.

Human pathology

Lu CL,Tang Y,Yang QP,Wang M,Zhao S,Bi CF,Jiang NG,Zhang WY,Liu JP,Xu X,Liu WP

MA5-11587 was used in immunohistochemistry to characterize seventeen clinical cases of hepatosplenic T-cell lymphoma

Thu Dec 01 00:00:00 EST 2011

Study of cellular immune response against Hepatitis E virus (HEV).

Journal of viral hepatitis

Prabhu SB,Gupta P,Durgapal H,Rath S,Gupta SD,Acharya SK,Panda SK

MA5-11587 was used in immunohistochemistry to investigate the immunopathology of hepatitis E virus infection

Mon Aug 01 00:00:00 EDT 2011

Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients.

Annals of dermatology

Lee DW,Yang JH,Lee SM,Won CH,Chang S,Lee MW,Choi JH,Moon KC

MA5-11587 was used in immunohistochemistry to study subcutaneous panniculitis-like T-cell lymphomas in Korean patients

Mon Aug 01 00:00:00 EDT 2011

Aristolochic acid nephropathy revisited: a place for innate and adaptive immunity?

Histopathology

Pozdzik AA,Berton A,Schmeiser HH,Missoum W,Decaestecker C,Salmon IJ,Vanherweghem JL,Nortier JL

MA5-11587 was used in immunohistochemistry to investigate the histological features of aristolochic acid nephropathy

Mon Mar 01 00:00:00 EST 2010

Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.

Journal of immunology (Baltimore, Md. : 1950)

Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT

MA5-11587 was used in immunohistochemistry to study the role of early control of immune activation in SIV infection of natural and non-natural hosts

Thu May 15 00:00:00 EDT 2008

Presence of HSV-1 immediate early genes and clonally expanded T-cells with a memory effector phenotype in human trigeminal ganglia.

Brain pathology (Zurich, Switzerland)

Derfuss T,Segerer S,Herberger S,Sinicina I,Hüfner K,Ebelt K,Knaus HG,Steiner I,Meinl E,Dornmair K,Arbusow V,Strupp M,Brandt T,Theil D

MA5-11587 was used in immunohistochemistry to investigate the distribution of HSV-1 immediate early genes in trigeminal ganglia and its influence on T cell function

Mon Oct 01 00:00:00 EDT 2007

Distinct time pattern of complement activation and cytotoxic T cell response in Guillain-Barré syndrome.

Brain : a journal of neurology

Wanschitz J,Maier H,Lassmann H,Budka H,Berger T

MA5-11587 was used in immunohistochemistry to study the dynamics of complement activation and cytotoxic T cell responses in Guillain-Barre syndrome

Mon Sep 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: C11; Cathepsin G-like 1; CTLA-1; CTSGL1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin 2; Fragmentin-2; granzyme 2; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GranzymeB; HLP; Human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; OTTHUMP00000028189; SECT; T-cell serine protease 1-3E

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Gene Aliases: CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; GRB; GZMB; HLP; SECT

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UniProt ID: (Human) P10144

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Entrez Gene ID: (Human) 3002

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Function(s)

serine-type endopeptidase activity protein binding serine-type peptidase activity protease serine protease protein modifying enzyme

Process(es)

apoptotic process protein processing cytolysis natural killer cell mediated cytotoxicity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway

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Granzyme B Monoclonal Antibody (GZB01)

Granzyme B Antibody (MA5-11587) in IHC (P)

Product Details

MA5-11587

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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