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Granzyme K Antibody (PA5-87719) in WB
Western blot analysis of Granzyme K in extracts of various cell lines. Samples were incubated with Granzyme K Polyclonal antibody (Product # PA5-87719) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87719
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-264 of human GZMK (NP_0020951)
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804366
Product Specific Information
Immunogen sequence: IIGGKEVSPH SRPFMASIQY GGHHVCGGVL IDPQWVLTAA HCQYRFTKGQ SPTVVLGAHS LSKNEASKQT LEIKKFIPFS RVTSDPQSND IMLVKLQTAA KLNKHVKMLH IRSKTSLRSG TKCKVTGWGA TDPDSLRPSD TLREVTVTVL SRKLCNSQSY YNGDPFITKD MVCAGDAKGQ KDSCKGDSGG PLICKGVFHA IVSGGHECGV ATKPGIYTLL TKKYQTWIKS NLVPPHTN; Positive Samples: Mouse liver, Rat liver; Cellular Location: Cytoplasmic granule
Target Information
Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Granzyme K; GrK; NK-Tryp-2; NK-tryptase-2; RNK-Tryp-2
Gene Aliases: Gzmk
UniProt ID: (Mouse) O35205, (Rat) P49864
Entrez Gene ID: (Mouse) 14945, (Rat) 29165
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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