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Invitrogen
CD268 (BAFF Receptor) Monoclonal Antibody (8A7), NovaFluor™ Blue 510, eBioscience™
FIGURE: 1 / 2
CD268 (BAFF Receptor) Antibody (H078T03B01-A) in Flow
Normal human peripheral blood cells were unstained (left) or stained with CD268 (BAFF Receptor) Monoclonal Antibody, NovaFluor Blue 510 (right). All cells were co-stained with CD19 Monoclonal Antibody, eFluor 450 (Product # 48-0199-42). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed... View More
Product Details
H078T03B01-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
8A7
Conjugate
Excitation/Emission Max
493/513 nm
View spectra
Form
Liquid
Concentration
0.4 µg/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Product Specific Information
Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells. The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression. Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression.
Applications Reported: The 8A7 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. It has also been reported for use in functional assays. (Please use Functional Grade purified 8A7, cat. 16-9117, in functional assays.).
Applications Tested: The 8A7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.4 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue) Laser
Target Information
Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13. 1-q13. 31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: B cell-activating factor receptor; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD268; Tumor necrosis factor receptor superfamily member 13C; tumor necrosis factor receptor superfamily, member 13C
Gene Aliases: BAFF-R; BAFFR; BR3; BROMIX; CD268; CVID4; prolixin; TNFRSF13C
UniProt ID: (Human) Q96RJ3
Entrez Gene ID: (Human) 115650
B cell homeostasis
adaptive immune response
positive regulation of germinal center formation
positive regulation of B cell proliferation
T cell costimulation
B cell costimulation
tumor necrosis factor-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of interferon-gamma biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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