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H3.3 K4M oncohistone mutant Antibody (712825) in WB
Western blot was performed using Anti-H3.3 K4M Recombinant Rabbit Polyclonal Antibody (Product # 712825) and a 17 kDa band corresponding to H3.3 K4M was observed in histone H3.3 K4M overexpressing HEK-293T cells confirming the specificity of H3.3 K4M antibody. Modified whole cell extracts (1% SDS) (30 µg lysate) from control (untransfected) (Lane 1), FLAG-tagged histone H3.3 K4M overexpressing HEK-293T cell... View More
Product Details
712825
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
16HCLC
Immunogen
Peptide corresponding to human H3.3K4M [aa2-aa9]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2890314
Product Specific Information
This antibody is predicted to react with Mouse, Rat and Bovine.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H3 histone, family 3B (H3.3B); H3 K4; H3 K4M; H3/b; H3/d; H3/f; H3/j; H3K4 mutant; H3K4M; Histone 3; Histone H3 K4M; Histone H3.3; Histone H3K4M
Gene Aliases: H3-3A; H3-3B; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; PP781
UniProt ID: (Human) P84243
Entrez Gene ID: (Human) 3020, (Human) 3021
chromatin silencing at rDNA
nucleosome assembly
DNA replication-independent nucleosome assembly
brain development
blood coagulation
response to hormone
positive regulation of cell growth
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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