Invitrogen

H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified

Advanced Verification

This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.

9 References

View all (20) H3K27me3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

H3K27me3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

H3K27me3 Antibody (MA5-11198) in ICC/IF

Immunofluorescence analysis of Tri-Methyl-Histone H3 (Lys27) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Monoclonal Antibody (Product # MA5-11198) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjug... View More

H3K27me3 Antibody in ChIP-Sequencing (ChIP-seq)

H3K27me3 Antibody in Peptide array (Array)

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Product Details

MA5-11198

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:1,600

View 2 publications 2 publications

ChIP assay (ChIP)

1-5 µg

View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

3 µg

Peptide Array (Array)

1:2,000

CUT&RUN (C&R)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.299.10

Immunogen

Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

102 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11000749

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with mono-methylated, di-methylated or tri-methylated histone H3 at Lys4, Lys9, Lys36 or Histone H4 at Lys20.

Click here for Master Lot linking information.

Target Information

H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heritable transcriptional defects from aberrations of nuclear architecture.

Nature

Papathanasiou S,Mynhier NA,Liu S,Brunette G,Stokasimov E,Jacob E,Li L,Comenho C,van Steensel B,Buenrostro JD,Zhang CZ,Pellman D

MA5-11198 was used in Immunocytochemistry to identify reductions in gene expression in chromosomes from micronuclei.

Sat Jul 01 00:00:00 EDT 2023

Dynamic antagonism between key repressive pathways maintains the placental epigenome.

Nature cell biology

Weigert R,Hetzel S,Bailly N,Haggerty C,Ilik IA,Yung PYK,Navarro C,Bolondi A,Kumar AS,Anania C,Brändl B,Meierhofer D,Lupiáñez DG,Müller FJ,Aktas T,Elsässer SJ,Kretzmer H,Smith ZD,Meissner A

MA5-11198 was used in Chromatin immunoprecipitation to find that the extra-embryonic epigenome reflects ongoing and dynamic de novo methyltransferase recruitment, which is continuously antagonized by Polycomb to maintain intermediate, locally disordered methylation.

Sat Apr 01 00:00:00 EDT 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

MA5-11198 was used in Chromatin immunoprecipitation to find that ADSCs-EVs transferred miR-22-3p to pneumonocytes and reduced apoptosis, inflammation, and oxidative stress through KDM6B/HMGA2, mitigating OSAHS-LI progression.

Wed Mar 01 00:00:00 EST 2023

Multi-CUT&Tag to simultaneously profile multiple chromatin factors.

STAR protocols

Gopalan S,Fazzio TG

MA5-11198 was used in CUT&RUN to develop a multi-CUTTag epigenetic profile protocol.

Fri Mar 18 00:00:00 EDT 2022

Epigenetic network of EZH2/SFRP1/Wnt in the epithelial-mesenchymal transition of laryngeal carcinoma cells.

Neoplasma

Chen W,Wang HT,Ji JF,Wang ZY,Shi T,Wu MH,Yu PJ

MA5-11198 was used in Western Blotting to explore the role and mechanism of EZH2 in the epithelial-mesenchymal transition (EMT) of laryngeal carcinoma (LGC) cells.

Sun May 01 00:00:00 EDT 2022

A common classification framework for histone sequence alterations in tumours: an expert consensus proposal.

The Journal of pathology

Leske H,Dalgleish R,Lazar AJ,Reifenberger G,Cree IA

MA5-11198 was used in Immunohistochemistry to provide an overview of tumour entities with sequence alterations of the H3-3A gene (HGNC ID = HGNC:4764), highlights the problems associated with the coexistence of these two nomenclatures, and proposes a standard for the reporting of histone sequence variants that allows an unambiguous description of these variants according to HGVS principles.

Tue Jun 01 00:00:00 EDT 2021

Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression.

Nature communications

Bakhoum MF,Francis JH,Agustinus A,Earlie EM,Di Bona M,Abramson DH,Duran M,Masilionis I,Molina E,Shoushtari AN,Goldbaum MH,Mischel PS,Bakhoum SF,Laughney AM

MA5-11198 was used in ChIP Assay, ChIP-Sequencing, Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide array, Western Blot, CUT&RUN to show that deregulation of PRC1 can promote tumor progression by inducing CIN and represents an opportunity for early therapeutic intervention.

Mon Sep 13 00:00:00 EDT 2021

Simultaneous profiling of multiple chromatin proteins in the same cells.

Molecular cell

Gopalan S,Wang Y,Harper NW,Garber M,Fazzio TG

MA5-11198 was used in ChIP Assay, ChIP-Sequencing, Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide array, Western Blot to describe multi-CUTTag, an adaptation of CUTTag that uses antibody-specific barcodes to simultaneously map multiple proteins in the same cells.

Thu Nov 18 00:00:00 EST 2021

Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat.

Frontiers in physiology

Beuzelin D,Pitard B,Kaeffer B

MA5-11198 was used in Chromatin immunoprecipitation to show extracellular miRNAs embedded in DOSP have a rapid impact on RNAi and on nuclear chromatin complexes depending on the daily rhythm.

Sat Nov 04 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K27; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3k27; H3Lys27me3; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K27me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K27; Tri-Methyl-H3 Lys27; Tri-Methyl-Histone H3 K27; Tri-Methyl-Histone H3 Lys27; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Mouse) Q8CGN9

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion histone

Process(es)

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified

H3K27me3 Antibody (MA5-11198) in ICC/IF

Product Details

MA5-11198

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Peptide Array (Array)

CUT&RUN (C&R)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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