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FIGURE: 1 / 9
H4K16ac Antibody (MA5-27794) in ICC/IF
Histone H4 (acetyl Lys16) antibody [GT1271] detects Histone H4 (acetyl Lys16) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [GT1271] (Product # MA5-27794) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
MA5-27794
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT1271
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4 (acetyl Lys16). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735098
Product Specific Information
Positive Control: HeLa (untreated), HeLa (0.4uM Trichostatin A for 18hr)
Predicted Reactivity: Xenopus laevis (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Click here for Master Lot linking information.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl Histone H4; Acetyl-H4 K16; Acetyl-H4 Lys16; Acetyl-Histone H4 K16; Acetyl-Histone H4 Lys16; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K16ac; H4K16; H4K16a; H4Lys16ac; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4 acetyl; Histone H4A; Histone H4K16; Histone H4K16ac; histone IV, family 2
Gene Aliases: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652
UniProt ID: (Human) P62805, (Mouse) Q811M0, (Mouse) P62806
Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504, (Mouse) 326619, (Mouse) 326620, (Mouse) 319158, (Mouse) 97122
chromatin silencing at rDNA
double-strand break repair via nonhomologous end joining
nucleosome assembly
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
DNA-templated transcription, initiation
telomere capping
gene silencing by RNA
telomere organization
CENP-A containing nucleosome assembly
cellular protein metabolic process
negative regulation of megakaryocyte differentiation
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
protein heterotetramerization
beta-catenin-TCF complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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