Invitrogen

HA Tag Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

12 References

View all (114) HA Tag antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 9

HA Tag Antibody (PA1-985) in WB

Western blot was performed using Anti-HA Tag Polyclonal Antibody (Product # PA1-985) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3, 40 µg (Lane 3), HA-H3, 20 µg (Lane 4), HA-H3, 10 µg (Lane 5), HA-H3, 5 µg (Lane 6), p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8 and 25 ng Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred ont... View More

HA Tag Antibody in Immunoprecipitation (IP)

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Product Details

PA1-985

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

5 µg

- -

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the residues Y P Y D V P D Y A of the HA epitope of influenza hemagglutinin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559366

Product Specific Information

PA1-985 detects HA tagged proteins. This antibody is specific for the HA tag and does not detect other proteins.

PA1-985 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of HA-tagged proteins.

The PA1-985 immunogen is a synthetic peptide corresponding to the residues Y P Y D V P D Y A of the HA epitope of influenza hemagglutinin.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Nature communications

Ge C,Tan J,Dai X,Kuang Q,Zhong S,Lai L,Yi C,Sun Y,Luo J,Zhang C,Zhu L,Wang B,Xu M

PA1-985 was used in Western Blot to identify DUSP22 as a key suppressor of NASH-HCC, and underscore the DUSP22-FAK axis as a promising therapeutic target for treatment of the disease.

Sat Oct 08 00:00:00 EDT 2022

Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets.

Methods in molecular biology (Clifton, N.J.)

Bacon K,Menegatti S,Rao BM

PA1-985 was used in Flow cytometry/Cell sorting to discuss how to assess the affinity and specificity of the isolated nanobodies.

Fri Feb 18 00:00:00 EST 2022

Subcellular specificity of cannabinoid effects in striatonigral circuits.

Neuron

Soria-Gomez E,Pagano Zottola AC,Mariani Y,Desprez T,Barresi M,Bonilla-Del Río I,Muguruza C,Le Bon-Jego M,Julio-Kalajzić F,Flynn R,Terral G,Fernández-Moncada I,Robin LM,Oliveira da Cruz JF,Corinti S,Amer YO,Goncalves J,Varilh M,Cannich A,Redon B,Zhao Z,Lesté-Lasserre T,Vincent P,Tolentino-Cortes T,Busquets-García A,Puente N,Bains JS,Hebert-Chatelain E,Barreda-Gómez G,Chaouloff F,Lohman AW,Callado LF,Grandes P,Baufreton J,Marsicano G,Bellocchio L

PA1-985 was used in Western Blotting to conclude that subcellular-specific CB1 receptor signaling within striatonigral circuits determines multimodal control of behavior.

Wed May 05 00:00:00 EDT 2021

Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)P₂ to direct migration of Drosophila embryonic germ cells.

Cell reports

Kim JH,Hanlon CD,Vohra S,Devreotes PN,Andrew DJ

PA1-985 was used in ChIP Assay, Immunoprecipitation, Western Blot to propose that Tre1 functions as a master helmsman in germ cell (GC) navigation by integrating extracellular signals to induce intracellular changes.

Tue Mar 02 00:00:00 EST 2021

Mapping the residue specificities of epigenome enzymes by yeast surface display.

Cell chemical biology

Waldman AC,Rao BM,Keung AJ

PA1-985 was used in ChIP Assay, Immunoprecipitation, Western Blot, Flow Cytometry to describe a versatile and scalable approach which enables efficient characterization of histone modifications without the need for recombinant protein production.

Thu Dec 16 00:00:00 EST 2021

Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping.

Methods in molecular biology (Clifton, N.J.)

Cruz-Teran CA,Bacon K,Rao BM

PA1-985 was used in Flow cytometry/Cell sorting to provide suggestions for testing the functionality and binding specificity of the soluble secreted proteins.

Wed Dec 16 00:00:00 EST 2020

Cul3 regulates cyclin E1 protein abundance via a degron located within the N-terminal region of cyclin E.

Journal of cell science

Davidge B,Rebola KGO,Agbor LN,Sigmund CD,Singer JD

PA1-985 was used in Western Blotting to describe a mechanism whereby N-terminally truncated cyclin E can avoid the Cul3-mediated degradation pathway.

Wed Nov 06 00:00:00 EST 2019

Sorbitol Modulates Resistance to Alternaria alternata by Regulating the Expression of an NLR Resistance Gene in Apple.

The Plant cell

Meng D,Li C,Park HJ,González J,Wang J,Dandekar AM,Turgeon BG,Cheng L

PA1-985 was used in Western Blotting to study the effect and mechanism of sorbitol on resistance to Alternata alternata.

Sun Jul 01 00:00:00 EDT 2018

Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment.

Development (Cambridge, England)

Eskova A,Chauvigné F,Maischein HM,Ammelburg M,Cerdà J,Nüsslein-Volhard C,Irion U

PA1-985 was used in Western Blotting to study the effect of the tissue environment on pigment cell behaviour and pattern formation in zebrafish.

Thu Jun 01 00:00:00 EDT 2017

Bioenergetic state regulates innate inflammatory responses through the transcriptional co-repressor CtBP.

Nature communications

Shen Y,Kapfhamer D,Minnella AM,Kim JE,Won SJ,Chen Y,Huang Y,Low LH,Massa SM,Swanson RA

PA1-985 was used in Western Blotting to elucidate the mechanisms by which glucose metabolism regulates pro-inflammatory NF-κB transcriptional activity.

Fri Sep 22 00:00:00 EDT 2017

Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency.

eLife

Southard S,Kim JR,Low S,Tsika RW,Lepper C

PA1-985 was used in immunohistochemistry to investigate the contribution of TEAD1 to muscle regeneration and pathology

Tue Oct 11 00:00:00 EDT 2016

Identification of PAN2 by quantitative proteomics as a leucine-rich repeat-receptor-like kinase acting upstream of PAN1 to polarize cell division in maize.

The Plant cell

Zhang X,Facette M,Humphries JA,Shen Z,Park Y,Sutimantanapi D,Sylvester AW,Briggs SP,Smith LG

PA1-985 was used in western blot to study the roles of PAN1 and PAN2 in the polarization of maize cell division

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin

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HA Tag Polyclonal Antibody

HA Tag Antibody (PA1-985) in WB

Product Details

PA1-985

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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