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HA Tag Antibody (740005MB) in WB
Western blot was performed using HA tag Recombinant Mouse Monoclonal Antibody (2-2.2.14), Biotin (Product # 740005MB) by loading modified whole cell extracts (1% SDS) of untransfected and transiently transfected HEK-293E lysates: Untransfected, 40 µg (Lane 1), Vector alone, 40 µg (Lane 2), HA-H3-DYKDDDDK, 40 µg (Lane 3), HA-H3-DYKDDDDK, 20 µg (Lane 4), HA-H3-DYKDDDDK, 10 µg (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Pr... View More
Product Details
740005MB
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
2-2.2.14
Immunogen
HA peptide YPYDVPDYA derivitized to ovalbumin.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3099401
Product Specific Information
HA tag recombinant mouse monoclonal antibody conjugated with Biotin specifically recognizes HA tag at both C and N-terminus of proteins.
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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