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HA Tag Antibody (740005MP555) in ICC/IF
Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3-DYKDDDDK transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with HA Tag Recombinant Mouse Monoclonal Antibody (2-2.2.14), Alexa Fluor™ Plus 555 (Product # 740005MP555, 1:400) and DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Alexa Fluor™ Plus 488 (Product # 701629RP488,1:400). Pane... View More
Product Details
740005MP555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
2-2.2.14
Immunogen
HA peptide YPYDVPDYA derivitized to ovalbumin.
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
HA tag recombinant mouse monoclonal antibody specifically recognizes HA tag at both C and N-terminus of proteins.
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This 2-2.2.14 antibody has been tested by western blot and flow cytometric analysis of HEK-293E cells were transiently transfected with HA Tag-protein constructs. This may be used for western blot at 2 µg/mL , 2.5 µg/mL for immunocytochemistry and for flow cytometry at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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