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FIGURE: 1 / 1
HAT1 Antibody (PA5-96526) in WB
Western blot analysis of HAT1 in extracts of various cell lines. Samples were incubated with HAT1 Polyclonal antibody (Product # PA5-96526) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96526
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 245-419 of human KAT1/KAT1/HAT1 (NP_0036331)
Conjugate
Form
Liquid
Concentration
0.38 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808328
Product Specific Information
Immunogen sequence: TPFQGQGHGA QLLETVHRYY TEFPTVLDIT AEDPSKSYVK LRDFVLVKLC QDLPCFSREK LMQGFNEDMA IEAQQKFKIN KQHARRVYEI LRLLVTDMSD AEQYRSYRLD IKRRLISPYK KKQRDLAKMR KCLRPEELTN QMNQIEISMQ HEQLEESFQE LVEDYRRVIE RLAQE; Positive Samples: MCF7, SW480, Jurkat, 293T, HeLa, HepG2, U-251MG, Mouse spleen, Rat testis; Cellular Location: Cytoplasm, Nucleus, Nucleus matrix, nucleoplasm
Target Information
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: histidine aminotransferase 1; Histone acetyltransferase 1; Histone acetyltransferase type B catalytic subunit; histone aminotransferase 1
Gene Aliases: 2410071B14Rik; AA536933; Hat-1; HAT1; KAT1
UniProt ID: (Human) O14929, (Mouse) Q8BY71, (Rat) Q5M939
Entrez Gene ID: (Human) 8520, (Mouse) 107435, (Rat) 296501
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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