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HCAR2 Antibody (MA5-51337) in WB
Western blot analysis of HCAR2 in extracts of PC-12 cells. Samples were incubated with HCAR2 Recombinant monoclonal antibody (Product # MA5-51337) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
MA5-51337
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7A3F2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GPR109A/HM74A/HCAR2 (NP_808219.1).
Conjugate
Form
Liquid
Concentration
0.59 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093101
Product Specific Information
Immunogen sequence: CRLMLFMLAM NRQGSIIFLT VVAVDRYFRV VHPHHALNKI SNRTAAIISC LLWGITIGLT VHLLKKKMPI QNGGANLCSS FSICHTFQWH EAMFLLEFFL P
Target Information
GPR109A (also known as HCA2, HM74a, and NIACR1) is a 42kDa G-protein coupled receptor that belongs to a family of three hydroxyl-carboxylic acid receptors, which also include GPR109B and GPR81. GPR109A has two known ligands: niacin and butyrate (or its derivative 3-hydroxybutyrate). The endogenous ligands for GPR109B and GPR81 are 3-hydroxyl-octanoic acid and 2-hydroxy-propanoate (lactate), respectively. GPR109A is expressed on adipocytes, neutrophils, Langerhans cells, keratinocytes, intestinal epithelial cells, and intestinal colonic macrophages. Niacin, also known as nicotinic acid or vitamin B3, is known to improve cholesterol metabolism (LDL/HDL ratio) and reduce inflammation. It has been demonstrated that this effect is mediated through GPR109A. Notably, GPR109A also has an essential role in suppressing inflammation and cancer in the colon. Both GPR109A ligands are produced by colonic commensal bacteria. In mice, GPR109A expression on intestinal epithelial cells and DCs/macrophages contributes to the suppression of colitis and colon cancer. In humans, GPR109A is silenced in colon carcinoma cells by DNA methylation, which increases inflammation in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 109A; G protein-coupled receptor 109B; G-protein coupled receptor 109; G-protein coupled receptor 109A; G-protein coupled receptor HM74; Hydroxycarboxylic acid receptor 2; interferon-gamma inducible gene, Puma-g; Niacin receptor 1; Nicotinic acid receptor; Protein PUMA-G; PUMA-G. GPR109; putative seven transmembrane spanning receptor
Gene Aliases: Gpr109; Gpr109a; Gpr109b; Hcar2; HM74; mHM74b; Niacr1; PUMA-G; Pumag
UniProt ID: (Mouse) Q9EP66
Entrez Gene ID: (Mouse) 80885
neutrophil apoptotic process
apoptotic process
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of neutrophil apoptotic process
purinergic nucleotide receptor signaling pathway
negative regulation of lipid catabolic process
positive regulation of adiponectin secretion
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