Invitrogen

HDAC1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-32443

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunocytochemistry (ICC/IF)

1:100
-

ChIP assay (ChIP)

2.5 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human HDAC-1 protein
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549912

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or breast carcinoma.

Target Information

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; Protein deacetylase HDAC1; Protein decrotonylase HDAC1; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2

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Gene Aliases: GON-10; HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1

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UniProt ID: (Human) Q13547, (Mouse) O09106

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Entrez Gene ID: (Human) 3065, (Mouse) 433759

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity activating transcription factor binding histone deacetylase binding transcription regulatory region DNA binding protein N-terminus binding NF-kappaB binding repressing transcription factor binding DNA binding chromatin binding transcription corepressor activity hydrolase activity histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) protein complex binding histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to amphetamine transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein deacetylation endoderm development circadian rhythm positive regulation of cell proliferation negative regulation of cell proliferation epidermal cell differentiation negative regulation of gene expression positive regulation of receptor biosynthetic process chromatin modification histone deacetylation hippocampus development neuron differentiation positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production circadian regulation of gene expression cellular response to oxidative stress odontogenesis of dentin-containing tooth positive regulation of tyrosine phosphorylation of Stat3 protein embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neuron apoptotic process negative regulation by host of viral transcription negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion rhythmic process positive regulation of oligodendrocyte differentiation negative regulation of androgen receptor signaling pathway hair follicle placode formation eyelid development in camera-type eye fungiform papilla formation histone H3 deacetylation histone H4 deacetylation negative regulation of canonical Wnt signaling pathway positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of type B pancreatic cell apoptotic process negative regulation of peptidyl-lysine acetylation negative regulation of intrinsic apoptotic signaling pathway chromatin remodeling methylation-dependent chromatin silencing blood coagulation negative regulation of myotube differentiation viral process regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly
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