Invitrogen

HDAC2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

20 Published Figures

37 References

View all (42) HDAC2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HDAC2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 29

HDAC2 Antibody (51-5100) in ICC/IF

Immunofluorescence analysis of HDAC2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HDAC2 Polyclonal Antibody (Product # 51-5100) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a d... View More

HDAC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC2 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

HDAC2 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

HDAC2 Antibody in Immunohistochemistry (IHC)

HDAC2 Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP)

HDAC2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

HDAC2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

HDAC2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with HDAC2 siRNA and reduction of signal was observed in Western Blot using HDAC2 Polyclonal Antibody (Product # 51-5100). {KD}

Product Details

51-5100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

5 µg

View 5 publications 5 publications

ChIP assay (ChIP)

Assay-dependent

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic 11 amino acid peptide derived from the C-terminus of the mouse HDAC2 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533908

Target Information

HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice.

EMBO molecular medicine

Farini A,Tripodi L,Villa C,Strati F,Facoetti A,Baselli G,Troisi J,Landolfi A,Lonati C,Molinaro D,Wintzinger M,Gatti S,Cassani B,Caprioli F,Facciotti F,Quattrocelli M,Torrente Y

51-5100 was used in ChIP Assay, ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to identify a potential role for the gut microbiota in the pathogenesis of DMD.

Wed Mar 08 00:00:00 EST 2023

HDAC1/2 Control Proliferation and Survival in Adult Epidermis and Pre‒Basal Cell Carcinoma through p16 and p53.

The Journal of investigative dermatology

Zhu X,Leboeuf M,Liu F,Grachtchouk M,Seykora JT,Morrisey EE,Dlugosz AA,Millar SE

51-5100 was used in Immunohistochemistry to find that homozygous epidermal codeletion of Hdac1 and Hdac2 in adult mouse epidermis causes reduced basal cell proliferation, apoptosis, inappropriate differentiation, and eventual loss of Hdac1/2-null keratinocytes.

Sat Jan 01 00:00:00 EST 2022

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in Immunohistochemistry (Paraffin)

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in Immunohistochemistry (Paraffin)

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in Immunohistochemistry (Paraffin)

Wed Mar 17 00:00:00 EDT 2021

Cancer Stem-Cell Marker CD44v9-Positive Cells Arise From Helicobacter pylori-Infected CAPZA1-Overexpressing Cells.

Cellular and molecular gastroenterology and hepatology

Tsugawa H,Kato C,Mori H,Matsuzaki J,Kameyama K,Saya H,Hatakeyama M,Suematsu M,Suzuki H

51-5100 was used in Western Blotting to determine the origin f CD44v9 positive cells.

Mon Jul 20 00:00:00 EDT 2020

PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart.

Experimental & molecular medicine

Yoon S,Kook T,Min HK,Kwon DH,Cho YK,Kim M,Shin S,Joung H,Jeong SH,Lee S,Kang G,Park Y,Kim YS,Ahn Y,McMullen JR,Gergs U,Neumann J,Kim KK,Kim J,Nam KI,Kim YK,Kook H,Eom GH

51-5100 was used in Immunoprecipitation to characterise the functional complex of histone deacetylase 2.

Thu Jul 26 00:00:00 EDT 2018

PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart.

Experimental & molecular medicine

Yoon S,Kook T,Min HK,Kwon DH,Cho YK,Kim M,Shin S,Joung H,Jeong SH,Lee S,Kang G,Park Y,Kim YS,Ahn Y,McMullen JR,Gergs U,Neumann J,Kim KK,Kim J,Nam KI,Kim YK,Kook H,Eom GH

51-5100 was used in Immunoprecipitation to characterise the functional complex of histone deacetylase 2.

Thu Jul 26 00:00:00 EDT 2018

Transient expression of ZBTB32 in anti-viral CD8+ T cells limits the magnitude of the effector response and the generation of memory.

PLoS pathogens

Shin HM,Kapoor VN,Kim G,Li P,Kim HR,Suresh M,Kaech SM,Wherry EJ,Selin LK,Leonard WJ,Welsh RM,Berg LJ

51-5100 was used in Chromatin immunoprecipitation to demonstrate that ZBTB32 is a vital transcription factor in CD8+ effector T cells for balanced regulation of effector versus memory infection responses.

Tue Aug 01 00:00:00 EDT 2017

Transient expression of ZBTB32 in anti-viral CD8+ T cells limits the magnitude of the effector response and the generation of memory.

PLoS pathogens

Shin HM,Kapoor VN,Kim G,Li P,Kim HR,Suresh M,Kaech SM,Wherry EJ,Selin LK,Leonard WJ,Welsh RM,Berg LJ

Tue Aug 01 00:00:00 EDT 2017

Spatial re-organization of myogenic regulatory sequences temporally controls gene expression.

Nucleic acids research

Harada A,Mallappa C,Okada S,Butler JT,Baker SP,Lawrence JB,Ohkawa Y,Imbalzano AN

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in ChIP assay

Fri Feb 27 00:00:00 EST 2015

Deacetylase inhibitors repress STAT5-mediated transcription by interfering with bromodomain and extra-terminal (BET) protein function.

Nucleic acids research

Pinz S,Unser S,Buob D,Fischer P,Jobst B,Rascle A

51-5100 was used in ChIP assay and western blot to test if deacetylase inhibitors target STAT5 or histone acetylation

Mon Apr 20 00:00:00 EDT 2015

Ctbp2 Modulates NuRD-Mediated Deacetylation of H3K27 and Facilitates PRC2-Mediated H3K27me3 in Active Embryonic Stem Cell Genes During Exit from Pluripotency.

Stem cells (Dayton, Ohio)

Kim TW,Kang BH,Jang H,Kwak S,Shin J,Kim H,Lee SE,Lee SM,Lee JH,Kim JH,Kim SY,Cho EJ,Kim JH,Park KS,Che JH,Han DW,Kang MJ,Yi EC,Youn HD

51-5100 was used in ChIP assay to investigate epigenetic changes in embryonic stem cell that control lineage commitment.

Sat Aug 01 00:00:00 EDT 2015

Inhibition of class I histone deacetylase activity represses matrix metalloproteinase-2 and -9 expression and preserves LV function postmyocardial infarction.

American journal of physiology. Heart and circulatory physiology

Mani SK,Kern CB,Kimbrough D,Addy B,Kasiganesan H,Rivers WT,Patel RK,Chou JC,Spinale FG,Mukherjee R,Menick DR

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in Western Blot

Mon Jun 01 00:00:00 EDT 2015

Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development.

Developmental dynamics : an official publication of the American Association of Anatomists

Cho OH,Mallappa C,Hernández-Hernández JM,Rivera-Pérez JA,Imbalzano AN

51-5100 was used in ChIP assay, immunocytochemistry, immunoprecipitation, and western blot to study the temporal regulation of MyoD in skeletal muscle differentiation

Thu Jan 01 00:00:00 EST 2015

Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development.

Developmental dynamics : an official publication of the American Association of Anatomists

Cho OH,Mallappa C,Hernández-Hernández JM,Rivera-Pérez JA,Imbalzano AN

51-5100 was used in ChIP assay, immunocytochemistry, immunoprecipitation, and western blot to study the temporal regulation of MyoD in skeletal muscle differentiation

Thu Jan 01 00:00:00 EST 2015

Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development.

Developmental dynamics : an official publication of the American Association of Anatomists

Cho OH,Mallappa C,Hernández-Hernández JM,Rivera-Pérez JA,Imbalzano AN

51-5100 was used in ChIP assay, immunocytochemistry, immunoprecipitation, and western blot to study the temporal regulation of MyoD in skeletal muscle differentiation

Thu Jan 01 00:00:00 EST 2015

Redox-sensitive gene-regulatory events controlling aberrant matrix metalloproteinase-1 expression.

Free radical biology & medicine

Bartling TR,Subbaram S,Clark RR,Chandrasekaran A,Kar S,Melendez JA

51-5100 was used in western blot to demonstrate that histone deacetylase-2 protein levels and its occupancy of the MMP-1 promoter are decreased in response to enforced manganese superoxide dismutase expression

Mon Sep 01 00:00:00 EDT 2014

Redox-sensitive gene-regulatory events controlling aberrant matrix metalloproteinase-1 expression.

Free radical biology & medicine

Bartling TR,Subbaram S,Clark RR,Chandrasekaran A,Kar S,Melendez JA

Mon Sep 01 00:00:00 EDT 2014

Transcriptional repression of ER through hMAPK dependent histone deacetylation by class I HDACs.

Breast cancer research and treatment

Plotkin A,Volmar CH,Wahlestedt C,Ayad N,El-Ashry D

51-5100 was used in western blot to study the relationship between hyperactivation of MAPK and the estrogen receptor in breast cancer cells

Mon Sep 01 00:00:00 EDT 2014

Epigenetic modifications induced by Blimp-1 Regulate CD8⁺ T cell memory progression during acute virus infection.

Immunity

Shin HM,Kapoor V,Guan T,Kaech SM,Welsh RM,Berg LJ

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in ChIP assay

Thu Oct 17 00:00:00 EDT 2013

Epigenetic modifications induced by Blimp-1 Regulate CD8⁺ T cell memory progression during acute virus infection.

Immunity

Shin HM,Kapoor V,Guan T,Kaech SM,Welsh RM,Berg LJ

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in ChIP assay

Thu Oct 17 00:00:00 EDT 2013

pVHL-mediated transcriptional repression of c-Myc by recruitment of histone deacetylases.

Molecules and cells

Hwang IY,Roe JS,Seol JH,Kim HR,Cho EJ,Youn HD

51-5100 was used in western blot to identify a novel mechanism for pVHL-mediated negative regulation of c-Myc transcription.

Wed Feb 01 00:00:00 EST 2012

Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells.

The Journal of biological chemistry

Aoyama T,Okamoto T,Fukiage K,Otsuka S,Furu M,Ito K,Jin Y,Ueda M,Nagayama S,Nakayama T,Nakamura T,Toguchida J

51-5100 was used in ChIP assay to explore the molecular mechanisms that regulate decreased ChM-I expression in mesenchymal stem cells

Fri Sep 24 00:00:00 EDT 2010

Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells.

The Journal of biological chemistry

Aoyama T,Okamoto T,Fukiage K,Otsuka S,Furu M,Ito K,Jin Y,Ueda M,Nagayama S,Nakayama T,Nakamura T,Toguchida J

51-5100 was used in ChIP assay to explore the molecular mechanisms that regulate decreased ChM-I expression in mesenchymal stem cells

Fri Sep 24 00:00:00 EDT 2010

Histone deacetylases facilitate sodium/calcium exchanger up-regulation in adult cardiomyocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chandrasekaran S,Peterson RE,Mani SK,Addy B,Buchholz AL,Xu L,Thiyagarajan T,Kasiganesan H,Kern CB,Menick DR

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in ChIP assay

Sun Nov 01 00:00:00 EDT 2009

Histone deacetylases facilitate sodium/calcium exchanger up-regulation in adult cardiomyocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chandrasekaran S,Peterson RE,Mani SK,Addy B,Buchholz AL,Xu L,Thiyagarajan T,Kasiganesan H,Kern CB,Menick DR

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in ChIP assay

Sun Nov 01 00:00:00 EDT 2009

Histone deacetylases facilitate sodium/calcium exchanger up-regulation in adult cardiomyocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chandrasekaran S,Peterson RE,Mani SK,Addy B,Buchholz AL,Xu L,Thiyagarajan T,Kasiganesan H,Kern CB,Menick DR

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in ChIP assay

Sun Nov 01 00:00:00 EDT 2009

Skeletal muscle specification by myogenin and Mef2D via the SWI/SNF ATPase Brg1.

The EMBO journal

Ohkawa Y,Marfella CG,Imbalzano AN

51-5100 was used in ChIP assay to elucidate how myogenin specifies the muscle phenotype

Wed Feb 08 00:00:00 EST 2006

The hTERT and hTERC telomerase gene promoters are activated by the second exon of the adenoviral protein, E1A, identifying the transcriptional corepressor CtBP as a potential repressor of both genes.

Neoplasia (New York, N.Y.)

Glasspool RM,Burns S,Hoare SF,Svensson C,Keith WN

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in Immunoprecipitation

Wed Jun 01 00:00:00 EDT 2005

The hTERT and hTERC telomerase gene promoters are activated by the second exon of the adenoviral protein, E1A, identifying the transcriptional corepressor CtBP as a potential repressor of both genes.

Neoplasia (New York, N.Y.)

Glasspool RM,Burns S,Hoare SF,Svensson C,Keith WN

Published figure using HDAC2 polyclonal antibody (Product # 51-5100) in Immunoprecipitation

Wed Jun 01 00:00:00 EDT 2005

Induction of HDAC2 expression upon loss of APC in colorectal tumorigenesis.

Cancer cell

Zhu P,Martin E,Mengwasser J,Schlag P,Janssen KP,Göttlicher M

51-5100 was used in immunohistochemistry - paraffin section to study the contribution of HDAC2 to the development of solid tumors

Sat May 01 00:00:00 EDT 2004

Class I histone deacetylase-selective novel synthetic inhibitors potently inhibit human tumor proliferation.

Clinical cancer research : an official journal of the American Association for Cancer Research

Park JH,Jung Y,Kim TY,Kim SG,Jong HS,Lee JW,Kim DK,Lee JS,Kim NK,Kim TY,Bang YJ

51-5100 was used in immunoprecipitation and western blot to assess the antitumor effects of novel hybrid synthetic histone deacetylase inhibitors on human cancer cells

Sun Aug 01 00:00:00 EDT 2004

Histone deacetylase inhibitors activate INK4d gene through Sp1 site in its promoter.

Oncogene

Yokota T,Matsuzaki Y,Miyazawa K,Zindy F,Roussel MF,Sakai T

Thu Jul 08 00:00:00 EDT 2004

Histone deacetylase inhibitors activate INK4d gene through Sp1 site in its promoter.

Oncogene

Yokota T,Matsuzaki Y,Miyazawa K,Zindy F,Roussel MF,Sakai T

Thu Jul 08 00:00:00 EDT 2004

Heregulin regulates the ability of the ErbB3-binding protein Ebp1 to bind E2F promoter elements and repress E2F-mediated transcription.

The Journal of biological chemistry

Zhang Y,Hamburger AW

Fri Jun 18 00:00:00 EDT 2004

Repression of E2F1-mediated transcription by the ErbB3 binding protein Ebp1 involves histone deacetylases.

Nucleic acids research

Zhang Y,Woodford N,Xia X,Hamburger AW

Tue Apr 15 00:00:00 EDT 2003

The histone deacetylase inhibitor valproic acid selectively induces proteasomal degradation of HDAC2.

The EMBO journal

Krämer OH,Zhu P,Ostendorff HP,Golebiewski M,Tiefenbach J,Peters MA,Brill B,Groner B,Bach I,Heinzel T,Göttlicher M

51-5100 was used in immunohistochemistry - paraffin section to demonstrate that poly-ubiquitination and proteasomal degradation are an isoenzyme-selective mechanism for the downregulation of HDAC2.

Tue Jul 01 00:00:00 EDT 2003

The histone deacetylase inhibitor valproic acid selectively induces proteasomal degradation of HDAC2.

The EMBO journal

Krämer OH,Zhu P,Ostendorff HP,Golebiewski M,Tiefenbach J,Peters MA,Brill B,Groner B,Bach I,Heinzel T,Göttlicher M

51-5100 was used in immunohistochemistry - paraffin section to demonstrate that poly-ubiquitination and proteasomal degradation are an isoenzyme-selective mechanism for the downregulation of HDAC2.

Tue Jul 01 00:00:00 EDT 2003

Sp1 and Sp3 recruit histone deacetylase to repress transcription of human telomerase reverse transcriptase (hTERT) promoter in normal human somatic cells.

The Journal of biological chemistry

Won J,Yim J,Kim TK

51-5100 was used in western blot to describe the systematic analyses of hTERT promoter to understand the transcriptional repression mechanism of the hTERT gene in normal human somatic cells.

Fri Oct 11 00:00:00 EDT 2002

Sp1 and Sp3 recruit histone deacetylase to repress transcription of human telomerase reverse transcriptase (hTERT) promoter in normal human somatic cells.

The Journal of biological chemistry

Won J,Yim J,Kim TK

51-5100 was used in western blot to describe the systematic analyses of hTERT promoter to understand the transcriptional repression mechanism of the hTERT gene in normal human somatic cells.

Fri Oct 11 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: HD2; hdac 2; hdac-2; Histone deacetylase 2; Protein deacylase HDAC2; transcriptional regulator homolog RPD3; YY1 transcription factor-binding protein; YY1-associated factor 1

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Gene Aliases: D10Wsu179e; HD2; HDAC2; mRPD3; RPD3; YAF1; Yy1bp

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UniProt ID: (Human) Q92769, (Mouse) P70288

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Entrez Gene ID: (Human) 3066, (Mouse) 15182

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II repressing transcription factor binding chromatin binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding heat shock protein binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity sequence-specific DNA binding poly(A) RNA binding NF-kappaB binding transcription factor activity, sequence-specific DNA binding hydrolase activity histone deacetylase activity (H3-K14 specific) chromatin DNA binding histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HDAC2 Polyclonal Antibody

HDAC2 Antibody (51-5100) in ICC/IF

Product Details

51-5100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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