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Invitrogen
HDAC3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 13
HDAC3 Antibody (PA5-29026) in ICC/IF
HDAC3 Polyclonal Antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 Polyclonal Antibody [C3], C-term (Product # PA5-29026) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-29026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 361 and 428 of Human HDAC3
Conjugate
Form
Liquid
Concentration
0.66 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546502
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, FaDu.
Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45
Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2
UniProt ID: (Human) O15379, (Mouse) O88895, (Rat) Q6P6W3
Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578
chromatin binding
transcription corepressor activity
histone deacetylase activity
protein binding
transcription factor binding
enzyme binding
cyclin binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
histone deacetylase binding
NF-kappaB binding
DNA binding
hydrolase activity
histone deacetylase activity (H3-K14 specific)
chromatin DNA binding
histone deacetylase activity (H3-K9 specific)
histone deacetylase activity (H4-K16 specific)
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
GTPase binding
NAD-dependent histone deacetylase activity (H3-K18 specific)
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
protein deacetylation
regulation of mitotic cell cycle
chromatin modification
histone deacetylation
regulation of protein stability
positive regulation of TOR signaling
negative regulation of interleukin-1 production
negative regulation of tumor necrosis factor production
circadian regulation of gene expression
regulation of multicellular organism growth
positive regulation of transcription factor import into nucleus
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of neuron apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
negative regulation of protein export from nucleus
spindle assembly
cellular response to fluid shear stress
positive regulation of type B pancreatic cell apoptotic process
negative regulation of cardiac muscle cell differentiation
negative regulation of myotube differentiation
response to nutrient levels
response to drug
histone H3-K9 modification
histone H3 deacetylation
histone H4 deacetylation
cellular response to mechanical stimulus
cellular response to parathyroid hormone stimulus
response to dexamethasone
circadian rhythm
negative regulation of apoptotic process
cellular lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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