Invitrogen

HELIOS Monoclonal Antibody (22F6), eFluor™ 450, eBioscience™

HELIOS Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

HELIOS Antibody (48-9883-42) in Flow

Intracellular staining of mouse lymph node cells with Anti-Mouse CD4 PE (Product # 12-0042-82), Anti-Mouse/Rat Foxp3 APC (Product # 17-5773-82), and Armenian Hamster IgG Isotype Control eFluor® 450 (Product # 48-4888-82) (left) or Anti-Helios eFluor® 450 (right) using the Foxp3/Transcription Factor Buffer Set and protocol (Product # 00-5523-00). CD4+ cells in the lymphocyte gate were used for analysis.

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Product Details

48-9883-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

22F6

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574136

Product Specific Information

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.

22F6 has been reported to cross react with rat Helios.

Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using Foxp3 Fixation and Permeabilization Buffers (Product # 00-5521-00). Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Activation-induced cytidine deaminase expression by thymic B cells promotes T-cell tolerance and limits autoimmunity.

iScience

Lombard-Vadnais F,Chabot-Roy G,Zahn A,Rodriguez Torres S,Di Noia JM,Melichar HJ,Lesage S

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Fri Jan 20 00:00:00 EST 2023

Interaction between tissue-dwelling helminth and the gut microbiota drives mucosal immunoregulation.

NPJ biofilms and microbiomes

Wang Y,Guo A,Zou Y,Mu W,Zhang S,Shi Z,Liu Z,Cai X,Zhu XQ,Wang S

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Sat Jun 24 00:00:00 EDT 2023

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

Piekarska K,Urban-Wójciuk Z,Kurkowiak M,Pelikant-Małecka I,Schumacher A,Sakowska J,Spodnik JH,Arcimowicz Ł,Zielińska H,Tymoniuk B,Renkielska A,Siebert J,Słomińska E,Trzonkowski P,Hupp T,Marek-Trzonkowska NM

48-9883-42 was used in Flow Cytometry to demonstrate that both direct and indirect contact with allogeneic ASCs improves therapeutic potential of Tregs (Regulatory T cells).

Mon Feb 14 00:00:00 EST 2022

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

48-9883-42 was used in Flow Cytometry to demonstrate that both direct and indirect contact with allogeneic ASCs improves therapeutic potential of Tregs (Regulatory T cells).

Mon Feb 14 00:00:00 EST 2022

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells.

Frontiers in immunology

Ramos-Ramírez P,Malmhäll C,Tliba O,Rådinger M,Bossios A

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Tue Oct 26 00:00:00 EDT 2021

Immune Dysregulation in Patients With Chromosome 18q Deletions-Searching for Putative Loci for Autoimmunity and Immunodeficiency.

Frontiers in immunology

Hogendorf A,Zieliński M,Constantinou M,Śmigiel R,Wierzba J,Wyka K,Wędrychowicz A,Jakubiuk-Tomaszuk A,Budzynska E,Piotrowicz M,Lipska-Ziętkiewicz BS,Kaczorowska E,Cieślikowska A,Kutkowska-Kaźmierczak A,Fijak-Moskal J,Kugaudo M,Kosińska-Urbańska M,Szadkowska A,Borowiec M,Niedźwiecki M,Trzonkowski P,Młynarski W

48-9883-42 was used in Flow Cytometry to determine the prevalence, mechanism, and genetic background of autoimmunity, immune deficiency, and allergy in a cohort of patients with 18q deletions.

Fri Dec 17 00:00:00 EST 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using HELIOS monoclonal antibody (Product # 48-9883-42) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

48-9883 was used in Flow cytometry/Cell sorting to reveal a function of B7-CD28 co-stimulation in shaping the T cell repertoire and limiting autoimmunity through both thymic clonal deletion and Treg cell generation.

Tue Dec 08 00:00:00 EST 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

Tue Dec 08 00:00:00 EST 2020

Regulatory T cell features in chronic granulomatous disease.

Clinical and experimental immunology

van de Geer A,Cuadrado E,Slot MC,van Bruggen R,Amsen D,Kuijpers TW

48-9883-42 was used in Flow Cytometry to investigate whether numerical or functional deficiencies exist in Tregs of patients with chronic granulomatous disease.

Thu Aug 01 00:00:00 EDT 2019

Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity.

Immunity

Cuadrado E,van den Biggelaar M,de Kivit S,Chen YY,Slot M,Doubal I,Meijer A,van Lier RAW,Borst J,Amsen D

48-9883 was used in Flow cytometry/Cell sorting to suggest that adaptation in signaling pathways protect Treg cell identity and present a resource for further research into Treg cell biology.

Tue May 15 00:00:00 EDT 2018

Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3⁺RORγt⁺ Regulatory T Cells.

Cell reports

Ogawa C,Bankoti R,Nguyen T,Hassanzadeh-Kiabi N,Nadeau S,Porritt RA,Couse M,Fan X,Dhall D,Eberl G,Ohnmacht C,Martins GA

48-9883-42 was used in Flow Cytometry to study the role of Blimp-1 as a crucial regulator of the Foxp3+RORγt+ regulatory T cell subset.

Tue Oct 02 00:00:00 EDT 2018

CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.

Cell reports

Shah SV,Manickam C,Ram DR,Kroll K,Itell H,Permar SR,Barouch DH,Klatt NR,Reeves RK

48-9883-42 was used in Flow Cytometry to provide a mechanism of virus-dependent alternative signaling that may explain the acquisition of adaptive features by primate NK cells and could be targeted for future vaccine or curative therapies.

Tue Dec 04 00:00:00 EST 2018

Suppressive IL-17A⁺Foxp3⁺ and ex-Th17 IL-17A^negFoxp3⁺ T_reg cells are a source of tumour-associated T_reg cells.

Nature communications

Downs-Canner S,Berkey S,Delgoffe GM,Edwards RP,Curiel T,Odunsi K,Bartlett DL,Obermajer N

48-9883 was used in Flow cytometry/Cell sorting to demonstrate the tumour-associated conversion of Th17 cells to Treg cells and identify transdifferentiation-associated markers.

Tue Mar 14 00:00:00 EDT 2017

Endothelium and NOTCH specify and amplify aorta-gonad-mesonephros-derived hematopoietic stem cells.

The Journal of clinical investigation

Hadland BK,Varnum-Finney B,Poulos MG,Moon RT,Butler JM,Rafii S,Bernstein ID

48-9883 was used in Flow cytometry/Cell sorting to investigate the critical niche components and resident signals required for hematopoietic stem cell induction and self-renewal ex vivo.

Fri May 01 00:00:00 EDT 2015

Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection.

PLoS pathogens

Leeansyah E,Svärd J,Dias J,Buggert M,Nyström J,Quigley MF,Moll M,Sönnerborg A,Nowak P,Sandberg JK

48-9883 was used in Flow cytometry/Cell sorting to show that IL-7 enhances the sensitivity of MAIT cells and induces the arming of effector functions.

Sat Aug 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios)

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Gene Aliases: A730095J18Rik; ANF1A2; HELIOS; IKZF2; Zfpn1a2; ZNF1A2; ZNFN1A2

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UniProt ID: (Human) Q9UKS7, (Mouse) P81183

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Entrez Gene ID: (Dog) 488510, (Human) 22807, (Mouse) 22779

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Function(s)

molecular_function transcription factor activity, sequence-specific DNA binding protein binding protein homodimerization activity transcription regulatory region DNA binding metal ion binding protein heterodimerization activity nucleic acid binding DNA binding C2H2 zinc finger transcription factor

Process(es)

transcription, DNA-templated biological_process positive regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HELIOS Monoclonal Antibody (22F6), eFluor™ 450, eBioscience™

HELIOS Antibody (48-9883-42) in Flow

Product Details

48-9883-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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