Invitrogen

HELIOS Monoclonal Antibody (22F6), Alexa Fluor™ 700, eBioscience™

HELIOS Antibody in Flow Cytometry (Flow)

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HELIOS Antibody (56-9883-42) in Flow

C57BL/6 mouse lymph node cells were stained with Foxp3 Monoclonal Antibody, eFluor 450 (Product # 48-5773-82) and Armenian Hamster IgG Isotype Control, Alexa Fluor 700 (Product # 56-4888-80) (left) or helios Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

56-9883-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

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Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Not Applicable

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

22F6

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784773

Product Specific Information

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.

22F6 has been reported to cross react with rat Helios.

Applications Reported: This 22F6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 22F6 antibody has been pre-diluted and tested by flow cytometric analysis of mouse lymph node cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Target Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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